NanoPhotometer
®
NP80/N60/N50/C40
User Guide Version 3.1
79
10. Use a lint-free laboratory wipe to clean both the sample window and
mirror prior to applying the next sample.
Note:
It can be helpful to apply the blank a second time and read it as a
sample to ensure a proper blank.
11. Apply sample to the sample window and press the sample button to
initiate the measurement.
C
ALCULATIONS
No calculations necessary: values are reported based on 10 mm path length.
The results show prominent peaks with wavelength and absorbance values. For cuvette
measurements it is possible to change to the %Transmittance mode. If a peak of interest is not
shown in the results the peak can be selected by clicking on the graph. The peak can then be
added to the results by tapping on the Add Peak button in the pop-up window.
M
ORE
A
PPS
:
A
BSORBANCE
R
ATIO
M
ETHOD
O
VERVIEW
In this mode, it is possible to determine simple absorbance ratios for a given sample by
measuring the absorbance at two wavelengths specified in the parameters of the method
relative to a blank.
M
EASUREMENT
P
ROTOCOL
1. Select the More Apps icon from the home screen and the
Absorbance/Ratio icon from the More Apps screen
2.
To change between NanoVolume and Cuvette application, use the
Change to Cuvette/NanoVolume button below the parameter area
(NP80/C40 only).
