NanoPhotometer
®
NP80/N60/N50/C40
User Guide Version 3.1
80
3.
For NanoVolume application
:
Select the dilution depending on the sample concentration
Note
: There is no automatic path length setting in this method. Select either a virtual
dilution of 15 (path length 0.67 mm) or of 140 (path length 0.07 mm).
For cuvette application:
Select the path length depending on the used cuvette.
Options are: 0.5 mm, 1 mm, 2 mm, 5 mm and 10 mm
For NanoVolume application with submicroliter cell (C40 only
):
Select Lid depending on your sample concentration (for concentration ranges of different
lids see page 39 Submicroliter Cell Measurements Basics). Required sample volume for
each lid is shown.
Note
: A new Blank is recommended when changing the dilution lid.
4. If it is desired to heat the sample to 37°C use the toggle switch to turn on
cell holder heater. When the cuvette holder has reached 37°C the toggle
color changes to green.
Note
: Only available for cuvette applications (NP80 and C40).
5. Enter desired wavelengths (
λ
1-1 and
λ
1-2) for ratio calculation. It is possible to measure
up to 20 absorbance/ratios simultaneously. More wavelengths for ratio calculation can be
added by selecting the Add Ratio button. Added ratios can be deleted with .
