NanoPhotometer C40 User Manual Download | Manualshive

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NanoPhotometer

®

NP80/N60/N50/C40

User Guide Version 3.1

109

9.

W

ARRANTY

Implen guarantees that the product supplied has been thoroughly tested to ensure that it meets
its published specification. The warranty included in the conditions of supply is valid for 12
months and is valid only if the product has been used according to the instructions supplied.
Implen or your supplier can accept no liability of loss or damage arising from the faulty or
incorrect use of this product

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Summary of Contents for C40

Page 1: ...NanoPhotometer NP80 N60 N50 C40 User Manual Version 3 1 Software Version 3 1 13220 ...

Page 2: ...C 47 CFR Part15 15 107 and 15 109 EN 301 489 1 V1 9 2 Radio and ancillary equipment for portable use portable equipment EUT Operating frequency range 2 4 2 4835 GHz EN 300 328 V1 8 1 Electromagnetic compatibility and Radio spectrum Matters ERM Wideband transmission systems Data transmission equipment operating in the 2 4 GHz ISM band and using wide band modulation techniques Harmonized EN covering...

Page 3: ... 21 SPECTROPHOTOMETER INSTALLATION 21 SAFETY INFORMATION 21 UNPACKING AND POSITIONING 22 SOFTWARE INSTALLATION 23 NPOS OVERVIEW 23 REQUIREMENTS AND COMPATIBILITY 23 INSTALLING SOFTWARE ON COMPUTER 24 INSTALLING NANOPHOTOMETER APP ON TABLET OR SMARTPHONE 25 FIRST STEPS AND CONFIGURATION WIZARD 26 PRINTER INSTALLATION 26 3 NANOPHOTOMETER BASICS 27 APPLICATIONS OVERVIEW 27 ICONS 29 BUTTONS 30 SIDE TA...

Page 4: ...OCOL 62 CALCULATIONS 66 KINETICS 66 METHOD OVERVIEW 66 MEASUREMENT PROTOCOL 66 CALCULATIONS 68 OD600 68 METHOD OVERVIEW 68 MEASUREMENT PROTOCOL 70 CALCULATIONS 72 MORE APPS 73 MORE APPS WAVELENGTH 73 METHOD OVERVIEW 73 MEASUREMENT PROTOCOL 73 CALCULATIONS 76 MORE APPS WAVESCAN 76 METHOD OVERVIEW 76 MEASUREMENT PROTOCOL 76 CALCULATIONS 79 MORE APPS ABSORBANCE RATIO 79 METHOD OVERVIEW 79 MEASUREMENT...

Page 5: ...Y 92 ABOUT 93 LANGUAGE 93 ILLUMINATION SAMPLE WINDOW 94 ENABLE NANOVOLUME 94 DYES 94 WARNING MESSAGES 95 BLANK CONTROL 95 SAMPLE QUALITY CONTROL 95 NETWORK 96 WIFI 97 NETWORK PRINTER 97 REPORT CONFIGURATION 98 6 TROUBLESHOOTING 99 SELF CALIBRATION TEST 99 7 ASSISTANCE 100 SUPPORT 100 REPORT PROBLEM 100 SOFTWARE MAINTENANCE 101 RESET 101 SOFTWARE UPDATE 101 CREATE LOG FILE 101 INSTALL PRINTER DRIVE...

Page 6: ...PLAATSEN 113 12 APPENDIX 3 INSTALLATION DU SPECTROPHOTOMÈTRE 114 INFORMATIONS DE SÉCURITÉ 114 DÉBALLAGE ET POSITIONNEMENT 115 13 APPENDIX 4 INSTALLATION SPECTROPHOTOMETER 117 SICHERHEITSINFORMATIONEN 117 AUSPACKEN UND PLATZIEREN 117 14 APPENDIX 5 INSTALLAZIONE DELLO SPETTROFOTOMETRO 119 INFORMAZIONI DI SICUREZZA 119 DISIMBALLAGGIO E COLLOCAZIONE 120 15 APPENDIX 6 INSTALACJA SPEKTROFOTOMETRU 121 IN...

Page 7: ...0 C40 User Guide Version 3 1 7 SÄKERHETSINFORMATION 126 UPPACKNING OCH PLACERING 127 18 APPENDIX 9 INSTALAÇÃO DE ESPECTROFOTÓMETRO 128 INFORMAÇÕES DE SEGURANÇA 128 DESEMBALAMENTO E POSICIONAMENTO 129 19 ALPHABETICAL APPENDIX 131 ...

Page 8: ... unmatched precision and accuracy without the need for dilutions Combined with our True Path Technology the system offers lifetime accuracy and precision without the need for maintenance or recalibration Note It is recommended to use a properly calibrated pipette with high quality tips to ensure delivery of appropriate sample volumes for nanovolume sample applications Sample Control is a quality c...

Page 9: ...NanoPhotometer NP80 N60 N50 C40 User Guide Version 3 1 9 NANOPHOTOMETER NP80 Item Number Touchscreen Battery Pack NP80 NP80 Touch NP80 Mobile ...

Page 10: ...NanoPhotometer NP80 N60 N50 C40 User Guide Version 3 1 10 NANOPHOTOMETER N60 Item Number Touchscreen Battery Pack N60 N60 Touch N60 Mobile ...

Page 11: ...NanoPhotometer NP80 N60 N50 C40 User Guide Version 3 1 11 NANOPHOTOMETER N50 Item Number Touchscreen Battery Pack N50 N50 Touch ...

Page 12: ...NanoPhotometer NP80 N60 N50 C40 User Guide Version 3 1 12 NANOPHOTOMETER C40 Item Number Touchscreen Battery Pack C40 C40 Touch C40 Mobile ...

Page 13: ...s 3 seconds initiate a hard reset Only activate a hard reset of the unit when necessary To avoid excess hard reboots it is recommended to power down the unit from the onboard touch screen by clicking on the power button in the bottom left corner INSTRUMENT BOTTOM VIEW NP80 N60 N50 C40 Model name device serial number and FCC ID are located on the identification plate on the bottom of the instrument...

Page 14: ...lation NanoPhotometer Power Adapter Power adapter for the NanoPhotometer and for charging the optional built in battery pack Note Use only the power adapter supplied with your instrument or a replacement part from the manufacturer or your supplier Dust Cover OPTIONAL ACCESSORIES Didymium Glass Filter C40 NP80 The certified didymium glass filter can be used to verify the wavelength and photometric ...

Page 15: ...noPhotometer N50 Submicroliter Cell C40 only The submicroliter cell provides the option of upgrading the NanoPhotometer C40 to a device that handles nanovolume samples as well as cuvette samples The sample volume range of the submicroliter cell ranges from a minimum of 0 3 µl to 5 0 µl with the path length options including 0 04 mm 0 1 mm 0 2 mm 1 mm and 2 mm which correspond to virtual dilution f...

Page 16: ...w The imported name can then be edited or replaced completely Barcode readers that have been tested and verified compatible 1D Honeywell Voyager 95X0 Single Line Laser Scanner Datalogic Touch65 1D 2D AGPtEK SC36 Honeywell Xenon 1900g DYMO Label Printer It is possible to connect a DYMO Label printer to the NanoPhotometer for direct printing on standard or cryo labels Recommended and tested printers...

Page 17: ... 3 1 17 Printout for DYMO LabelWriter 4XL Printout for DYMO LabelWriter 450 Note The printouts are optimized for the label size of the DYMO LabelWriter 4XL All other DYMO printers can be used However the font size will be zoomed to utilized paper size ...

Page 18: ...e We recommend using FAT FAT32 formatted 2 0 USB flash drives Encrypted USB flash drives are not compatible with the NanoPhotometer Note Cordless Bluetooth mice are not supported Use wired mice only Note Connect mouse and keyboard before starting the NanoPhotometer USB B There is a USB B port located on the rear panel of the instrument which is compatible with the USB cable provided to connect the...

Page 19: ...limited to 40 measurements per dataset Larger datasets can be saved on the NanoPhotometer itself HDMI There is an HDMI port located on the rear panel of the NanoPhotometer which is compatible with HDMI 1 4 cables or better to connect the NanoPhotometer to HDMI compatible monitors Note The maximum HDMI cable length is 5 meters NANOPHOTOMETER SPECIFICATIONS NanoVolume Performance Detection Range dsD...

Page 20: ...martphone and tablet Software Compatibility Windows 7 32 and 64 bit Windows 8 32 and 64 bit OS X iOS and Android Min Requirement Smartphone Tablet 4 inch screen Apple iPad 2 iPhone 5 and iOS 6 Android Phone OS version 4 4 Android Tablet OS version 5 0 Quad core 1 2 GHz with 1 GB RAM General Specifications Size 200 mm x 200 mm x 140 mm Weight 3 8 5 2 kg depending on configuration Operating Voltage ...

Page 21: ...osure when measuring without lid Do not use damaged power cords accessories and other peripherals with your NanoPhotometer Use only the delivered and specified power supply charger Do not expose the NanoPhotometer to strong magnetic electrical fields water chemicals or any type of liquid as heavy rain or moisture Do not put the instrument into fire as it may swell or explode battery Do not store a...

Page 22: ...nge 10 40 C If the cuvette heating is used the range is 10 30 C If the instrument is subjected to extreme temperature changes it may be necessary to allow the instrument to equilibrate Turn the instrument off and then on again once thermal equilibrium has been established 2 3 hours Maximum relative humidity non condensing of 80 and up to 31 C decreasing linearly to 50 at 40 C The instrument must b...

Page 23: ...er until the NanoPhotometer NPOS software is installed on the computer REQUIREMENTS AND COMPATIBILITY The NPOS user interface is designed that all features can be operated by using a touchscreen If the software is installed on a computer without touchscreen the user interface can be operated by using a keyboard and a mouse Before starting the installation process ensure the device is compatible wi...

Page 24: ... the update on your computer 2 Launch the NPOS installation file NanoPhotometer msi and follow the installation routine The installer should be logged in with full administration rights If you have insufficient privileges installation may fail If in doubt consult your PC administrator 3 Open the NPOS software and choose the preferred connection For USB connect the NanoPhotometer with the provided ...

Page 25: ...itch off the browser safety This may interfere with the NPOS running on your computer INSTALLING NANOPHOTOMETER APP ON TABLET OR SMARTPHONE The NanoPhotometer App can be installed as an application on tablets and smartphones with compatible Android and iOS operating systems The NanoPhotometer App is available for free download in the app store Apple Store and Google Play Store 1 Download and insta...

Page 26: ...otometer with previous software versions printer driver installation is necessary 1 Switch NanoPhotometer on home screen 2 Update to newest firmware version Update Process a Copy NPOS bin to a USB flash drive into the root folder b Insert the USB flash drive into your NanoPhotometer c Select Assistance Software Maintenance d Click on Update e Wait until the NanoPhotometer reboots Note Always updat...

Page 27: ... can be performed with 10 mm 5 mm 2 mm 1 mm and 0 5 mm path length quartz glass or plastic cuvettes with a center height of 8 5 mm APPLICATIONS OVERVIEW The NanoPhotometer comes with pre programmed applications as well as the ability to create custom applications To select a method click tap on the corresponding icon and the method will open immediately Method Icons Description Nucleic Acids Conce...

Page 28: ...a secondary method screen Wavelength Define one or multiple wavelength between 200 900 nm N50 200 650 nm for absorbance measurements Wavescan Define desired full scan range anywhere between 200 900 nm N50 200 650 nm Concentration Define extinction coefficient for automatic concentration calculations Absorbance Ratio Define two wavelengths absorbance ratio calculation Standard Curve Create a standa...

Page 29: ...y Delete Deletes added functions in parameter empties input windows Delete Data Opens a delete dialog pop up Email Data Opens an email dialog pop up computer and tablet versions only Save Data Opens a save dialog pop up Print Data Opens a print dialog pop up only shown when DYMO HP printer is successfully installed and connected Add Folder Adds a new folder to the directory Manage Data Opens a dia...

Page 30: ...e applications Selecting the Change to Cuvette button activates the cuvette compartment opens the sliding cover and switches the red arrow LED on Only cuvette measurements are possible when this mode is selected Selecting the Change to NanoVolumebutton while the cuvette port is activated will deactivate the cuvette compartment closes the sliding cover and switches the LED off and only nanovolume m...

Page 31: ... parameter area it is possible to define all necessary parameters for a measurement as well as turn on cuvette mode and initiate cuvette heating The default measurement screen includes the parameter area as open It is automatically hidden when starting either a blank or sample measurement by clicking on the Blank or Sample button It is also possible to hide the parameter area by tapping the parame...

Page 32: ...easurements will be automatically overlaid To change the overlaid graphs use the tick boxes in the table Note It is only possible to overlay up to 30 graphs in a chart If more than 30 data are selected a message will appear that says More than 30 samples have been selected Only 30 will be shown in graph Note The overlay button is not available on the NanoPhotometer and smartphones only on tablets ...

Page 33: ...ork please disconnect the installed USB printer Note The print icon is only shown if a printer is available Note It is only possible to operate one printer at a time Do not connect more than one printer to the NanoPhotometer Note The print option is not available in the smartphone apps Auto Print If the auto print function is enabled each measurement will be printed directly after the measurement ...

Page 34: ...always all measurements saved no selection possible Save as Type With the save type option it is possible to specify the file type for saving File type options include Excel PDF and Implen Document Source IDS It is possible to save different file formats simultaneously Note IDS files can only be saved on the NanoPhotometer and on a USB flash drive It is not possible to save IDS files from opened d...

Page 35: ...the file Allowed characters are A Z a z 0 9 _ Note Blank character is not allowed Storage Selection option to choose where you would like the data to be saved The options include NanoPhotometer USB flash drive if connected and Control Device If Control Device is selected the data will be transferred to the device that is currently connected with the NanoPhotometer Note It is not possible to save I...

Page 36: ...2 0 µl Standard Cuvette applications can be performed with 10 mm 5 mm 2 mm 1 mm and 0 5 mm path length quartz glass or plastic cuvettes with a center height of 8 5 cm Note NanoVolume application in the NanoPhotometer C40 is only available with the optional accessory submicroliter cell from Implen NANOVOLUME MEASUREMENT BASICS N50 N60 NP80 1 Select a method depending on your sample and set the para...

Page 37: ... to enter a sample name for each sample in the input window enter sample name Note Allowed characters are A Z a z 0 9 _ Note Blank character is not allowed 8 Raise the sample arm and pipette the appropriate amount of sample solution onto the illuminated sample window Upon completion of measurement raise sample arm clean the surfaces and apply the next sample Note Parameter setting Volume 1 2 µl ad...

Page 38: ...below the parameter area NP80 only 3 Add a blank solution to a cuvette and ensure that the filling volume is sufficient to allow light path to pass through the solution 4 Insert cuvette into the cell holder 5 Initiate a blank measurement with the blank button Upon completion of measurement remove the cuvette 6 Add sample to a cuvette and ensure that the sample volume is sufficient to allow light t...

Page 39: ...on We guarantee our submicroliter cell specifications only for Implen submicroliter cells which are aligned to the NanoPhotometer C40 by Implen 3 Set the parameter for the measurement Choose the dilution lid depending on your sample concentration Lid dsDNA ng µl BSA mg ml absorbance range 10 mm path 5 optional 2 375 0 05 7 5 10 5 750 0 15 22 0 10 15 50 25 3 750 0 70 108 0 50 150 100 optional 50 7 ...

Page 40: ... of measurement remove the lid clean the surfaces and apply the next sample SAMPLE HANDLING TIPS Nanovolume Methods The NanoPhotometer includes an integrated vortexer N60 NP80 only to assure sample homogeneity It is recommended to vortex every sample right before the measurement The sample window is illuminated NP80 N60 N50 only with a low energy red light to assist with accurate sample applicatio...

Page 41: ... on the cuvette type used it is necessary that the light passes through the sample for accurate measurements Center height is 8 5 mm Note The cuvette holder is not removable Do not pour any cleaning solution into the cuvette holder as larger amounts of liquids can get into the instrument and cause damage Solvent Compatibility N50 N60 NP80 C40 with submicroliter cell Most solvents typically used in...

Page 42: ...word secured WiFi connection To access transfer data from the NanoPhotometer network drive via LAN connection the serial number or the NanoPhotometer IP needs to be entered in the address bar of the Windows Explorer e g M80798 or Assigned IP Address Using a USB cable connection 192 168 7 1 will provide access and for password secured WiFi connections 192 168 8 1 will need to be entered in the addr...

Page 43: ...harge the battery at this status If the battery is not charged there will be a second warning message when the battery is empty and several beep sounds before the instrument is automatically shutting down within 10 to 15 seconds Note Unsaved data can be found in the auto save folder in Stored Methods If the NanoPhotometer is shut down during a measurement process the data of this measurement are l...

Page 44: ...0 nm ratios Further it is possible to determine the degree of labeling of nucleic acids with probes including fluorescent dyes Sample Control gives useful information about sample conditions It recognizes air bubbles sample impurities turbidity lint residues and potential contaminations If Sample Control detects any inconstancy an alert icon is shown in the result table area A click on the alert i...

Page 45: ...7 mm path length for samples with concentrations 420 ng µl dsDNA For cuvette application Select the path length depending on the used cuvette Options are 0 5 mm 1 mm 2 mm 5 mm and 10 mm For NanoVolume application with submicroliter cell C40 only Select a Lid depending on your sample concentration for concentration ranges of different lids see also page 39 Submicroliter Cell Measurements Basics Req...

Page 46: ...ions are 320 nm 340 nm It is also possible to add any wavelength in the range of 220 350 nm 7 8 Air bubble recognition is disabled as a default Set to on it detects air bubbles lint residues and bad sample conditions of the sample Note Lint residues and bad sample conditions are detected even if the air bubble recognition is set to off 9 For dye labelled samples tick the dye labels in the list whi...

Page 47: ...asurement and select blank to initiate the reading Note The illumination of the sample window can be switched off in the preferences 12 Use a lint free laboratory wipe to clean both the sample window and mirror prior to applying the next sample Note It could be helpful to apply the blank a second time and read it as a sample to ensure a proper blank 13 Apply sample to the sample window and press t...

Page 48: ...ound wavelength 10 mm path Đ Manual dilution factor εnuc Extinction coefficient nucleic acid factor in ng cm µl Table 1 Nucleic acids extinction coefficients εnuc Type εnuc dsDNA 50 ng cm µl ssDNA 37 ng cm µl RNA 40 ng cm µl miRNA 33 ng cm µl Oligo 33 ng cm µl miRNA Seq calculated via extinction coefficient of constituent nucleotides entered Oligo Seq calculated via extinction coefficient of const...

Page 49: ...c acid factor in ng cm µl cfdye Dye dependent correction factor at 260 nm Đ Manual dilution factor Note Dye correction is only available for single dye selection Dye Concentration For dye labeled nucleic acids the concentration of the dye is calculated using a modified form of the Beer Lambert equation For these calculations the instrument considers the absorption maximum of the dye the dye specif...

Page 50: ... ABKG cfdye Amax dye ABKG With background correction without dye correction FOI 324 5 Amax dye ABKG εdye 106 A260 ABKG εnuc Without background correction and with dye correction FOI 324 5 Amax dye εdye 106 A260 cfdye Amax dye εnuc Without background correction and without dye correction FOI 324 5 Amax dye εdye 106 A260 εnuc FOI Frequency of Incorporation dye per 1 000 bases A260 Absorbance at 260 ...

Page 51: ...50 000 0 05 NP80 N60 C40 Cy5 5 675 250 000 0 05 NP80 N60 N50 C40 Oyster 500 503 78 000 0 29 NP80 N60 N50 C40 Oyster 550 553 150 000 0 05 NP80 N60 N50 C40 Oyster 556 560 155 000 0 03 NP80 N60 C40 Oyster 645 649 220 000 0 05 NP80 N60 C40 Oyster 650 653 200 000 0 04 NP80 N60 C40 Oyster 656 660 200 000 0 04 NP80 N60 N50 C40 Texas Red 593 85 000 0 23 Ratios Reactions utilizing nucleic acids often requi...

Page 52: ... shown in the results table area A click on the alert icon shows additional information The ranges for acceptable ratio values can be defined in preferences The ratios are calculated with or without background correction according to if the background correction is activated during the measurements or not as follows Without background correction 260 280 ratio A260 A280 260 230 ratio A260 A230 With...

Page 53: ... determines protein concentration by performing calculations based on specific ε values either pre programmed in the instrument or entered manually by the user Extinction coefficient ε values at 280 nm vary greatly for different proteins due to their particular aromatic amino acid content Fixed ε values are pre programmed in the software for certain proteins see Table 3 However if the protein of i...

Page 54: ... the right side Options are BSA SA Mouse SA Human IgG Mouse IgG Human IgE Human Lysozyme OD1 Molar Extinction Coefficient and Extinction Coefficient For Mol Ext Coefficient enter Molecular Weight default 66 400 g mol and Mol Ext Coefficient default 44 289 M 1 cm 1 For Ext Coefficient enter Ext Coefficient default 0 69 l g cm 4 For NanoVolume application Select the volume of sample to be applied No...

Page 55: ...hen changing the dilution lid 5 The background correction is enabled at 320 nm as a default It is possible to disable the background correction with the toggle switch Selection options are 320 nm 340 nm It is also possible to add any wavelength in the range of 220 350 nm 6 Air bubble recognition is disabled as a default When enabled it detects air bubbles lint residues and poor conditions of the s...

Page 56: ...d dye is not available in the list please go to preferences and add a custom dye to the dye list There is an option for dye correction which can be enabled disabled with a toggle switch Note Dye correction is only available for single dye selection 9 Option to set calculate a dilution factor for manual diluted samples 10 Apply the blank ddH20 or buffer to the illuminated sample window for the refe...

Page 57: ...tton to initiate the measurement CALCULATIONS Protein UV280 Concentration The protein concentration in the Protein UV method is calculated with the absorbance value of the sample at 280 nm along with the extinction coefficient defined by the user The protein concentration is calculated with or without background correction as follows With background correction C A280 ABKG εprot Ð Without backgroun...

Page 58: ...factor at 280 nm see Table 4 The dye concentration is calculated with or without background dye correction for are as follows With background and with dye correction C A280 ABKG cfdye Amax dye ABKG εprot Ð With background and without dye correction C A280 ABKG εprot Ð Without background and with dye correction C A280 cfdye Amax dye εprot Ð Without background and without dye correction C A280 εprot...

Page 59: ...th εdye Extinction coefficient of dye in M 1 cm 1 Ð Dilution factor Degree of Labeling DOL DOL is the degree of labeling based on the average number of dye molecules coupled to a protein molecule The degree of labeling can be determined from the absorption spectrum of the labeled antibody with or without background dye correction as follows With background and with dye correction DOL Amax dye ABKG...

Page 60: ...0 C40 Alexa Fluor 546 554 104 000 0 12 NP80 N60 N50 C40 Alexa Fluor 555 555 150 000 0 08 NP80 N60 N50 C40 Alexa Fluor 568 577 91 300 0 46 NP80 N60 N50 C40 Alexa Fluor 594 590 73 000 0 56 NP80 N60 C40 Alexa Fluor 647 650 239 000 0 03 NP80 N60 C40 Alexa Fluor 680 679 184 000 0 05 NP80 N60 C40 Alexa Fluor 790 785 260 000 0 08 NP80 N60 N50 C40 Cy3 550 150 000 0 05 NP80 N60 C40 Cy5 649 250 000 0 05 NP8...

Page 61: ...ange at the appropriate wavelength for each assay and compared against a standard curve prepared by serial dilution of a protein standard of known concentration A linear linear zero or 2nd order regression analysis of the calibration standard data points is calculated by the NanoPhotometer A correlation coefficient R2 in the range of 0 95 to 1 00 indicates a good fit to a straight line Bradford As...

Page 62: ...th a standard protein at 750 nm this is usually BSA bovine serum albumin Note Detailed protocols are customarily supplied with these assay kits and must be closely followed to ensure that accurate results are obtained MEASUREMENT PROTOCOL 1 Select protein assays icon from home screen 2 To change between NanoVolume and cuvette application use the Change to Cuvette NanoVolume button below the parame...

Page 63: ...es of different lids see page 39 Submicroliter Cell Measurements Basics Required sample volume for each lid is shown Note A new Blank is recommended when changing the dilution lid 5 If it is desired to heat the sample to 37 C use the toggle switch to turn on cell holder heater When the cuvette holder has reached 37 C the toggle color changes to green Note Only available for cuvette applications NP...

Page 64: ...ions are linear regression zero regression forces the straight line through the origin and 2nd order regression 8 Select Unit 9 Add up to 20 Concentrations by clicking on the Add Concentration button Added concentrations can be deleted with Enter the concentrations of the standard curve ...

Page 65: ...ble to exclude single measurements from the curve calculation by switching the toggle switch off Note After the first sample measurement the standard curve can no longer be altered 12 Once a Standard curve is created or loaded it will be used for concentration calculations of measured samples It may be necessary to do a blank measurement 13 Apply sample and press the sample button to initiate the ...

Page 66: ...onding changes in the concentration of reactants or products as the reaction progresses The rate of many chemical reactions can be markedly accelerated by the presence of catalysts which remain chemically intact during the reaction Catalysts in the case of biochemical reactions are generally represented by enzymes which are specialized protein catalysts However a few examples of special reactions ...

Page 67: ...ts Basics Required sample volume for each lid is listed to the right Note A new Blank is recommended when changing the dilution lid 4 If it is desired to heat the sample to 37 C use the toggle switch to turn on the cell holder heater When the cuvette holder has reached 37 C the toggle color changes to green Note Only available for cuvette applications NP80 and C40 5 Default wavelength λ is 340 nm ...

Page 68: ...ange of parameters is only possible before starting the kinetic readings Save and delete data is only available after the kinetic session is stopped Note Auto print and cryo label print is not available in Kinetics CALCULATIONS All absorbance values are normalized to a 10 mm path A0 absorbance of start value 10 mm path An absorbance of actual value time n 10 mm path dA absorbance of actual value a...

Page 69: ... sample Therefore if results from different spectrophotometers are to be compared they must be normalized first using appropriate calibration curves A calibration curve can be constructed by comparing measured OD 600 to expected OD 600 Expected OD 600 is determined by counting cell number using an alternative technique for example microscope slide method and converting to OD 600 using the rule of ...

Page 70: ...to Cuvette NanoVolume button below the parameter area NP80 C40 only 3 For Cuvette Application Select the path length depending on the used cuvette Options are 0 5 mm 1 mm 2 mm 5 mm and 10 mm For NanoVolume Application Select the dilution depending on the sample concentration Note There is no automatic path length setting in this method Select either a virtual dilution of 15 path length 0 67 mm or ...

Page 71: ...se the toggle switch to turn on cell holder heater When the cuvette holder has reached 37 C the toggle color changes to green Note Only available for cuvette applications NP80 and C40 5 Default wavelength is 600 nm but the wavelength can be changed in the range of 200 900 nm N50 200 650 nm depending on the application 6 Toggle switch cells ml is disabled as default Enable cells ml to get the cells...

Page 72: ...mples 10 Insert cuvette with the reference sample and select blank button to initiate the measurement 11 Insert cuvette with the sample and select the sample button to initiate the measurement CALCULATIONS OD600 A600 Ð cf OD600 Optical density at 600 nm A600 Absorbance at 600 nm 10 mm path Ð Dilution factor cf Correction factor for spectrophotometer Cells ml A600 Ð cf multiplier A600 Absorbance at...

Page 73: ...nd transmittance Trans only in cuvette mode of a sample at specific wavelengths It is possible to add up to 20 different wavelengths MEASUREMENT PROTOCOL 1 Select the More Apps icon from the home screen and the Wavelength icon from the More Apps screen 2 To change between NanoVolume and Cuvette application use the Change to Cuvette NanoVolume button below the parameter area NP80 C40 only 3 For Nan...

Page 74: ...ch to turn on cell holder heater When the cuvette holder has reached 37 C the toggle color changes to green Note Only available for cuvette applications NP80 and C40 5 Enter desired wavelength λ to be measured It is possible to measure up to 20 wavelengths simultaneously More wavelength λ options can be added by selecting the Add Wavelength button Added wavelength can be deleted with 6 Baseline co...

Page 75: ...ples 9 Apply the blank ddH20 or buffer to the illuminated sample window for the reference measurement and select blank to initiate the reading Note The illumination of the sample window can be switched off in preferences 10 Use a lint free laboratory wipe to clean both the sample window and mirror prior to applying the next sample Note It can be helpful to apply the blank a second time and read it...

Page 76: ...the absorbance A and transmittance T of a sample with respect to a reference at a specific wavelength Transmittance is the ratio of light intensity remaining after it has passed through the sample I to the initial incident light intensity I0 𝑇𝑇 𝐼𝐼 𝐼𝐼0 𝑇𝑇 𝐼𝐼 𝐼𝐼0 100 MORE APPS WAVESCAN METHOD OVERVIEW Using the wavescan application it is possible to obtain the complete spectral scan for a defined wa...

Page 77: ...m 5 mm and 10 mm For NanoVolume application with submicroliter cell C40 only Select Lid depending on your sample concentration for concentration ranges of different lids see page 39 Submicroliter Cell Measurements Basics Required sample volume for each lid is shown Note A new Blank is recommended when changing the dilution lid 4 If it is desired to heat the sample to 37 C use the toggle switch to ...

Page 78: ... nm 604 nm 650 nm 770 nm N A N50 and 823 nm N A N50 Option to enter any wavelength between 200 nm and 900 nm N50 650 nm 7 Option to smooth the graph with different boxcars Options Off 1 boxcar 11 default 2 boxcar 21 and 3 boxcar 61 8 Option to set calculate a dilution factor for manual diluted samples 9 Apply the blank ddH20 or buffer to the illuminated sample window for the reference measurement ...

Page 79: ...s it is possible to change to the Transmittance mode If a peak of interest is not shown in the results the peak can be selected by clicking on the graph The peak can then be added to the results by tapping on the Add Peak button in the pop up window MORE APPS ABSORBANCE RATIO METHOD OVERVIEW In this mode it is possible to determine simple absorbance ratios for a given sample by measuring the absor...

Page 80: ... sample concentration for concentration ranges of different lids see page 39 Submicroliter Cell Measurements Basics Required sample volume for each lid is shown Note A new Blank is recommended when changing the dilution lid 4 If it is desired to heat the sample to 37 C use the toggle switch to turn on cell holder heater When the cuvette holder has reached 37 C the toggle color changes to green Not...

Page 81: ...tions Off 1 boxcar 11 default 2 boxcar 21 and 3 boxcar 61 8 Option to set calculate a dilution factor for manual diluted samples 9 Apply the blank ddH20 or buffer to the illuminated sample window for the reference measurement and select blank to initiate the reading Note The illumination of the sample window can be switched off in the preferences 10 Use a lint free laboratory wipe to clean both th...

Page 82: ...ated for a sample by determining the absorbance at a specific wavelength relative to a reference The concentration is then obtained by multiplying the measured absorbance by a specific factor This factor may be known in advance and entered by the user or it may be calculated by the instrument by measuring a set of standard standard curve method with known concentrations to create a standard curve ...

Page 83: ...le concentration for concentration ranges of different lids see page 39 Submicroliter Cell Measurements Basics Required sample volume for each lid is shown Note A new Blank is recommended when changing the dilution lid 4 If it is desired to heat the sample to 37 C use the toggle switch to turn on cell holder heater When the cuvette holder has reached 37 C the toggle color changes to green Note Onl...

Page 84: ...ult Enabling the baseline corrections shows a list with different wavelength options 377 nm 604 nm 650 nm 770 nm N A N50 and 823 nm N A N50 Option to enter any wavelength between 200 nm and 900 nm N50 650 nm 9 Option to smooth the graph with different boxcars Options Off 1 boxcar 11 default 2 boxcar 21 and 3 boxcar 61 ...

Page 85: ...ct blank to initiate the reading Note The illumination of the sample window can be switched off in the preferences 12 Use a lint free laboratory wipe to clean both the sample window and mirror prior to applying the next sample Note It can be helpful to apply the blank a second time and read it as a sample to ensure a proper blank 13 Apply sample to the sample window and press the sample button to ...

Page 86: ...tify samples of the same type with unknown concentrations This application provides an extremely useful tool with which to integrate expedite and simplify the measurement and calculations involved in determining the concentration of analytes in unknown samples If a zero concentration standard is required include it in the number of standards to be entered using a reagent blank and entering 0 00 fo...

Page 87: ...or each lid is shown Note A new Blank is recommended when changing the dilution lid 4 If it is desired to heat the sample to 37 C use the toggle switch to turn on cell holder heater When the cuvette holder has reached 37 C the toggle color changes to green Note Only available for cuvette applications NP80 and C40 5 Baseline correction is set to off as default Enabling the baseline corrections show...

Page 88: ...otometer NP80 N60 N50 C40 User Guide Version 3 1 88 6 Select the curve fit type Options are linear regression zero regression forces the straight line through the origin and 2nd order regression 7 Select Unit ...

Page 89: ...ill be used for concentration calculations in the method It might be necessary to do a blank measurement 11 Apply sample and press the sample button to initiate the measurement Note Once the sample measurement is initiated it is not possible to make changes to the standard curve CALCULATIONS Concentration is determined via the absorbance values provided by the standard curve based on the curve fit...

Page 90: ...n the left side the subfolders of that storage folder and on the right side the individual files On the right side of the screen all saved result files of the selected folder are shown and can be opened by a long or double click Folders can be deleted renamed moved or copied by clicking on the icon It is also possible to delete rename move or copy files by clicking on the icon The file path of the...

Page 91: ...older shows on the left side the subfolders of the storage folder and on the right side the method files On the right side of the screen all saved method files of the selected folder are shown and can be opened by a long or double click On the top of the right area the file path of the selected folder is shown New folders can be created by clicking on Folders can be deleted renamed moved or copied...

Page 92: ...neral in the Preferences menu opens a window to the right of the preferences menu with the following options Display built in display only About WiFi Access Point and Illumination Sample Window NP80 N60 N50 or Enable NanoVolume C40 DATE AND TIME Within the Date and Time it is possible to set the actual date and time Change the date and or time by clicking on the date time field The selected time i...

Page 93: ... following languages English Chinese French German Japanese Portuguese Russian or Spanish The language is changed for the user interface printouts and saved Excel and PDF files Note The change will affect all methods Note It is possible to work in users desired language and change the selected language for Excel and PDF Printouts to English This option can be selected with the toggle switches Exce...

Page 94: ...s To toggle between the nucleic acid and protein list click on the Nucleic Acid Protein buttons in the header Each dye has either a lock icon in front of the dye name indicating that the dye is locked and cannot be changed or a delete symbol The delete option is only available for unlocked and not preprogrammed dyes Selecting a dye name opens a new screen with the dye information dye name absorban...

Page 95: ...cm 1 and dye dependent correction factor There is a toggle switch available to lock the dye to prevent deleting a dye from the dye list accidentally WARNING MESSAGES BLANK CONTROL Toggle switch to switch on off the Blank Control of the NanoPhotometer Note Blank Control is available for all NanoVolume methods for NP80 N60 N50 SAMPLE QUALITY CONTROL It is possible to change the upper and the lower l...

Page 96: ...t the DHCP protocol is selected Connect the NanoPhotometer with the Ethernet and the IP is automatically set during the startup of the NanoPhotometer If no IP Address is listed for Current IPv4 click on the save button to search for an available IP address For static IP configuration select Static in the Configure IPv4 dropdown and enter the IPv4 address netmask and gateway then confirm with the s...

Page 97: ...ad or iPhone to the NanoPhotometer To set up a WiFi network select Connect to WiFi Network in the dropdown Select an available WiFi network from the dropdown list or choose other for hidden networks and enter the password Confirm with the save icon NETWORK PRINTER It is possible to print via Airprint IPP compatible printers supporting PDF format in the network Note IPP version 2 2 is required and ...

Page 98: ...he highest priority and will be the printer used by default when selecting Print on NanoPhotometer In order to print utilizing a printer on the network please disconnect the installed USB printer REPORT CONFIGURATION In report configuration it is possible to define the table columns of PDF files and A4 printouts This feature is available for the methods Nucleic Acids and Protein UV To toggle betwe...

Page 99: ... If the instrument does not pass the test a message window will appear explaining the reason for the failed test along with the recommended solution If the ok button is selected the window is closed and the home screen will be shown with an error message at the top This error message will be displayed on top of all other screens and all performed measurements including the saved files of those mea...

Page 100: ...able on computer and tablet versions only When report a problem is highlighted in the left side assistance menu the right side will have a form to fill out with information including first name last name phone number email and country A dropdown menu provides the option to select the problem type and includes the following selections error message software firmware measurements and other It is als...

Page 101: ... before updating Note Make sure that the NanoPhotometer is connected to power and that the power connection is not interrupted during the update It is recommended to do the update via the built in touchscreen If the touchscreen is not available it is also possible to do the update via computer or tablet In this case it is necessary to relaunch the NanoPhotometer software after the reboot of the Na...

Page 102: ...the Install Driver for Printers button 5 Wait until the following message is shown Installed driver for printer successfully 6 Go to home screen 7 Connect DYMO HP printer via USB cable 8 Wait for 30 seconds 9 DYMO HP printer is ready to use Note In order to connect a printer to the NanoPhotometer please do so from the Home Screen Printers will not properly function if USB connection of printer is ...

Page 103: ...e contact the Implen team directly Europe Asia South Pacific Middle East and Africa Implen GmbH Schatzbogen 52 81829 München Germany Phone 49 89 72637180 Fax 49 89 726371854 Email support implen de www implen de North and South America Implen Inc Unit 104 31194 La Baya Drive Westlake Village CA 91362 USA Phone 1 818 748 6400 Telefax 1 818 449 0416 Email support implen com Website www implen com ...

Page 104: ...ion certification using filters traceable to international standards during production and quality control certified engineers and calibrated test equipment approved to ISO 9001 standard automatic self diagnostic calibration test during start of the NanoPhotometer result is documented in each data output file and possibility to save a Implen Document Source IDS file no data manipulation possible R...

Page 105: ...ir bubble lint residue or a bad sample like e g a turbid sample Check sample clean the pedestal and mirror in the lid thoroughly and reapply sample carefully To avoid air bubbles apply sample by reverse pipetting Another control device is currently connected Message is shown on a control device when another control device is currently connected with an open measurement session Please close method ...

Page 106: ...der is always empty Files are saved in the Download Folder of the Android tablet smartphone directory Instrument connected to control device Do you really want to intercept This message is shown on the NanoPhotometer when a method is open on another control device Click on OK and the measurements session is interrupted and the data are saved in the auto save folder Lint residue or bad sample Pleas...

Page 107: ... has a higher software version than the installed one NPOS software is not downward compatible Initialization failed problem with optical path Please contact Implen customer support Check if the cuvette port is empty and or clean the nanovolume pedestal and the mirror in the head with 70 EtOH and distilled water then reboot the NanoPhotometer If the message still appears please create a log file a...

Page 108: ...ed Please contact Implen customer support Make sure that the sample name is NPOS bin without any additions or extensions like from multiple downloads otherwise rename file to be NPOS bin exactly NPOS bin file must be in the root folder of the USB flash drive Try the update a second time if it still fails please create a log file and send it to Implen Customer Support Stored method result was creat...

Page 109: ...sted to ensure that it meets its published specification The warranty included in the conditions of supply is valid for 12 months and is valid only if the product has been used according to the instructions supplied Implen or your supplier can accept no liability of loss or damage arising from the faulty or incorrect use of this product ...

Page 110: ...pům kapalin jako je například silný déšť nebo vlhkost Nevhazujte přístroj do ohně protože se může zdeformovat nebo explodovat baterie Neskladujte ani nepoužívejte přístroj v blízkosti jakéhokoli tepelného zdroje především při teplotách nad 60 C nebo ve výbušné atmosféře Nenechávejte přístroj NanoPhotometer na klíně nebo jakékoli části těla zabráníte tak nepohodlí nebo poranění vlivem působení žáru...

Page 111: ... při 40 C Přístroj musí být umístěn na stabilním rovném povrchu s nosností 4 až 5 kg a musí být zajištěná volná cirkulace vzduchu okolo přístroje Zajistěte aby po zapnutí přístroje žádné materiály nebránily cirkulaci vzduchu Vyhněte se přímému slunečnímu záření protože může docházet k vyblednutí částí přístroje a poškození plastových dílů Přístroj byste měli umístit tak aby v případě nouze bylo mo...

Page 112: ... vermelde voeding lader Stel de NanoPhotometer niet bloot aan sterke magnetische elektrische velden water chemische stoffen of andere vloeistoffen zoals zware regen of vocht Plaats het instrument niet in vuur Dit kan opzwellen of ontploffen batterij Gebruik niet op of nabij een warmtebron in het bijzonder temperaturen boven 60 C of in een explosieve omgeving Plaats uw NanoPhotometer niet op uw sch...

Page 113: ...ochtigheid niet condenserend 80 en tot 31 C lineair afnemend tot 50 bij 40 C Het instrument moet op een stabiel vlak oppervlak worden geplaatst dat 4 5 kg kan dragen en moet zo worden geplaatst dat lucht vrij rond het instrument kan stromen Zorg ervoor dat de luchtcirculatie niet wordt geblokkeerd wanneer het instrument is ingeschakeld Voorkom rechtstreeks zonlicht omdat dit delen van het instrume...

Page 114: ... d alimentation chargeur fourni N exposez pas le NanoPhotometer à de puissants champs magnétiques électriques à de l eau à des produits chimiques ou à d autres types de liquide par exemple à de fortes précipitations ou à de l humidité N exposez pas l instrument au feu car il pourrait gonfler ou exploser batterie Ne stockez pas et n utilisez pas l instrument à proximité d une source de chaleur tout...

Page 115: ...10 40 C si vous utilisez le chauffage de la cuvette la plage de températures est de 10 30 C Si l instrument est soumis à des changements de température extrêmes il peut s avérer nécessaire de le laisser s équilibrer Mettez l instrument hors tension puis remettez le sous tension une fois que l équilibre thermique est atteint environ 2 à 3 heures Humidité relative maximale sans condensation de 80 à ...

Page 116: ...rie de vérifications d autodiagnostic Veuillez contacter immédiatement le fournisseur d origine si vous rencontrez des difficultés techniques ou de manipulation d échantillons Remarque si cet équipement est utilisé d une manière non conforme à ce qui est spécifié ou dans des conditions environnementales inadaptées à son fonctionnement sécurisé la protection qu il offre peut s en trouver impactée c...

Page 117: ...e integrierte Batterie explodieren kann Nicht in der Nähe von Wärmequellen aufstellen bzw verwenden insbesondere keinen Temperaturen über 60 C aussetzten Betreiben sie das NanoPhotometer nicht auf Ihrem Schoß oder in der Nähe eines Körperteils um Unannehmlichkeiten oder Verletzungen durch Hitzeeinwirkung zu verhindern Stellen Sie keine Gegenstände auf dem NanoPhotometer ab NanoPhotometer mit Akku ...

Page 118: ...Gerät sollte so positioniert sein dass im Falle eines Notfalls der Stromstecker leicht identifiziert und entfernt werden kann Beim Transport des Gerätes nicht am optionalen Display oder Hebelarm tragen sondern immer am Hauptgehäuse Für den Betrieb des NanoPhotometers darf ausschließlich das 60W Netzteil Kabel welches von Implen geliefert wurde verwendet werden 90 W mit Akku Pack Stromversorgung 90...

Page 119: ...orti campi elettrici e o magnetici acqua sostanze chimiche o qualsiasi tipo di liquido ad esempio pioggia fitta o umidità Non porre lo strumento sul fuoco poiché può gonfiarsi o esplodere la batteria Non conservarlo e non utilizzarlo in prossimità di fonti di calore specificamente a temperature maggiori di 60 C o in un atmosfera esplosiva Il NanoPhotometer non deve essere poggiato sulle gambe o st...

Page 120: ...o su una superficie stabile e orizzontale in grado di sostenere un peso di 4 5 kg e tale da consentire la libera circolazione dell aria intorno allo strumento Assicurarsi che quando lo strumento è acceso non vi siano materiali a ostruire la circolazione dell aria Non esporre ai raggi solari diretti per evitare lo scolorimenti di parti dello strumento e o danni alle parti in plastica L apparecchiat...

Page 121: ...tarczonego w zestawie i zgodnego ze specyfikacją zasilacza lub ładowarki Nie wolno narażać urządzenia NanoPhotometer na działanie mocnych pól magnetycznych i elektrycznych wody chemikaliów ani jakichkolwiek płynów takich jak mocne opady atmosferyczne lub wilgoć Urządzenia nie wolno wrzucać do ognia ponieważ może napęcznieć lub eksplodować akumulator Urządzenia nie wolno przechowywać ani używać w p...

Page 122: ...e kuwety zakres ten wynosi 10 30 C W przypadku ekstremalnych zmian temperatury może być konieczne zrównoważenie temperatury urządzenia W tym celu należy je wyłączyć i włączyć ponownie po osiągnięciu równowagi termicznej po około 2 3 godzinach Maksymalna wilgotność względna bez kondensacji wynosi 80 w temperaturze do 31 C i zmniejsza się liniowo do 50 w temperaturze 40 C Urządzenie należy umieścić ...

Page 123: ...nicznych lub z próbką należy niezwłocznie skontaktować się z pierwotnym dostawcą urządzenia Uwaga Eksploatacja urządzenia w sposób niezgodny ze specyfikacją lub w warunkach niesprzyjających bezpiecznemu działaniu może zmniejszyć skuteczność jego zabezpieczeń i doprowadzić do unieważnienia gwarancji ...

Page 124: ...os intensos ni tampoco a agua productos químicos o ningún otro tipo de líquido como lluvia o humedad elevada No arroje el instrumento al fuego pues podría inflamarse o explotar la batería No lo guarde ni lo utilice en las proximidades de fuentes de calor en especial a temperaturas superiores a 60 C o en una atmósfera explosiva No se apoye el NanoPhotometer en el regazo ni cerca de ninguna parte de...

Page 125: ...ondensación del 80 y hasta 31 C con reducción lineal hasta 50 a 40 C El instrumento debe colocarse sobre una superficie estable y plana que pueda soportar 4 5 kg y que permita que el aire circule con libertad alrededor del instrumento Asegúrese de que ningún material reduzca la circulación del aire con la unidad encendida Evite la luz solar directa pues podría decolorar algunas partes del instrume...

Page 126: ...tometer för starka magneter elektriska fält vatten kemikalier eller någon typ av vätska som kraftigt regn eller fukt Lägg inte instrumentet i eld det kan då svälla och explodera batteriet Förvara inte och använd inte heller i närheten av någon typ av värmekälla särskilt inte temperaturer över 60 C eller i en explosiv atmosfär För att förhindra obehag av eller skada från värmeexponering ska din Nan...

Page 127: ...ande 80 och upp till 31 C linjärt minskande till 50 vid 40 C Instrumentet måste placeras på en stabil jämn yta som kan bära 4 5 kg och tillåter att luft fritt kan cirkulera runt instrumentet Kontrollera när den är påslagen att inget material minskar luftcirkulationen Undvik direkt solljus eftersom det kan bleka delar av instrumentet och orsaka skada på delar som är tillverkade av plast Utrustninge...

Page 128: ...ricas excessivas água produtos químicos ou qualquer outro tipo de líquido como a chuva forte ou a humidade Não submeta o instrumento a fogo dado que pode dilatar ou explodir bateria Não guarde nem use o instrumento na proximidade de qualquer tipo de fonte de calor especialmente a temperaturas superiores a 60 C ou em ambientes explosivos Não use o NanoPhotometer sobre o seu colo ou regaço nem junto...

Page 129: ...sligue o instrumento e volte a ligar depois de alcançar o equilíbrio térmico 2 3 horas A humidade relativa máxima não condensação é de 80 e até 31 C reduzindo progressivamente até 50 a 40 C O instrumento deve ficar posicionado numa superfície nivelada e estável e que suporte 4 5 kg e que permita que o ar circule livremente em redor do instrumento Ao ligar o instrumento certifique se de que não exi...

Page 130: ... 130 Nota Se este equipamento for utilizado de forma não especificada ou em condições ambientais não adequadas para o funcionamento em segurança a proteção proporcionada pelo equipamento pode ficar comprometida e a garantia do instrumento pode ser anulada ...

Page 131: ...ssay 61 Biuret Assay 61 Blank Control 8 95 Bradford Assay 61 Buttons 30 C Cleaning 105 Compatibility 23 Solvent 41 Concentration 82 Calculations 85 Measurements Protocol 82 Connecting Cable 14 Connectivity 18 Contact 103 Cryo Label 16 33 Custom Apps 90 Cuvette 37 41 D Data Transfer 42 Date and Time 92 Delete Data 35 Didymium Glass Filter 14 DiluCell 15 Display 92 DYMO Printer 16 E Edit 32 Excel 35...

Page 132: ...Protocol 70 On Off Switch 9 10 11 12 13 P Parameter area 31 PDF 35 Positioning 22 Collocazione 120 Instalación 125 Plaatsen 113 Placering 127 Platzieren 117 Posicionamento 129 Positionnement 115 Umiejscowienie 122 Umístění 111 Power Adapter 14 Power Button 13 Preferences 92 Blank Control 95 Dyes 94 General 92 Language 93 legal 102 Log File 101 Network 96 97 Printer Driver 102 Sample Quality Contro...

Page 133: ... Calibration Test 99 Side Tab Bar 31 Software Reset 101 Update 101 Solvent Compatibility 41 Standard Curve 86 Calculations 89 Measurements Protocol 86 Standard Solution 15 Stored Methods 91 Stored Results 90 Submicroliter Cell 15 30 39 T Table area 31 Touch Screen 104 Trademarks 102 Troubleshooting 99 U Unpacking 22 Auspacken 117 Déballage 115 Desembalaje 125 Desembalamento 129 Disimballaggio 120 ...

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