NanoPhotometer
®
NP80/N60/N50/C40
User Guide Version 3.1
39
S
UBMICROLITER
C
ELL
M
EASUREMENTS
B
ASICS
(C40)
The Implen submicroliter cell can be used with the NanoPhotometer
®
C40. Insert the
submicroliter cell to the cell holder with the cell windows facing the light beam. We recommend
facing the Implen logo to the right side. The light beam is directed from BACK to FRONT as
indicated with the white arrows.
Note
: For best performance insert the submicroliter cell always in the same direction.
1. Select a method depending on your sample.
2. Enable the NanoVolume application with the “Change to NanoVolume” button below the
parameter area.
Note
: If there is no “Change to NanoVolume” button available in the method go to
preferences/general and enable the NanoVolume option. We guarantee our submicroliter
cell specifications only for Implen submicroliter cells which are aligned to the
NanoPhotometer
®
C40 by Implen.
3. Set the parameter for the measurement. Choose the dilution lid depending on your sample
concentration.
Lid
dsDNA (ng/µl)
BSA (mg/ml)
absorbance range
10 mm path
5 (optional)
2 – 375
0.05 – 7.5
10
5 – 750
0.15 – 22
0.10 – 15
50
25 – 3,750
0.70 – 108
0.50 – 150
100 (optional)
50 – 7,500
1.45 – 217
1.00 – 150
250 (optional)
125 – 18,750
3.63 – 543
2.50 – 375
Note:
A new Blank is recommended when changing the dilution lid.
4. Ensure that the sample window and the mirror in the dilution lid are clean.
5. Pipette the appropriate sample volume (blank solution) onto the center of the measuring
window. The required volumes are also shown in the parameter area of the software.
Lid
Sample
volume
Path length
Dilution
5 (optional)
3.5 – 5 µl
2 mm
1:5
10
1 – 3 µl
1 mm
1:10
50
0.3 – 2 µl
0.2 mm
1:50
100 (optional)
0.3 – 2 µl
0.1 mm
1:100
250 (optional)
0.3 – 2 µl
0.04 mm
1:250
