NanoPhotometer
®
NP80/N60/N50/C40
User Guide Version 3.1
68
3,600 seconds (N50: 10–3,600 sec.), depending on the duration time.
Enter the delay time in seconds before the first measurement is taken. Possible delay time
is between 0–3,600 seconds, depending on the duration time.
Note
: A maximum of 500 samples is possible. Please consider this when choosing the
duration and interval time.
7. Insert cuvette with the reference sample and select blank button to initiate
the measurement.
8. Insert cuvette with the sample and select the sample button to initiate the
measurements. Once the kinetic is started the Blank button turns to a
Pause/Continue button and the Sample button to a Stop button.
Note
: While the kinetics is running it is not possible to change the parameters, save data or
delete data. Change of parameters is only possible before starting the kinetic readings. Save
and delete data is only available after the kinetic session is stopped.
Note
: Auto print and cryo label print is not available in Kinetics.
C
ALCULATIONS
All absorbance values are normalized to a 10 mm path.
A
0
= absorbance of start value (10 mm path)
A
n
= absorbance of actual value time n (10 mm path)
dA
= absorbance of actual value – absorbance of start value
Slope
= linear regression fit of all actual measurement points
Final A = absorbance of final value
R
2
= R
2
=
∑
(O
i
-E
i
)
2
E
i
n
i=1
[O
i
= observed slope value; E
i
= expected slope value]
OD600
M
ETHOD
O
VERVIEW
The growth of bacteria in liquid culture media is commonly monitored by measuring the optical
density at 600 nm (OD600) in small samples taken from the cultures. OD600 measurements are
typically used to determine the stage of growth of the bacterial culture, thereby ensuring that
cells are harvested at an optimum point that corresponds to an appropriate density of live cells.
