NanoPhotometer
®
NP80/N60/N50/C40
User Guide Version 3.1
40
Note
: Do not overfill the well.
Note:
A new Blank is recommended when changing the dilution lid.
6. For measurements, ensure that the lid fits exactly onto the positioning supports mounted to
the body of the cell and initiate a blank measurement with the blank button.
Note
: Possible UV exposure. Only initiate a measurement when the lid is closed.
7. Clean the measurement window and mirror in the lid with a slightly wet lint-free tissue. Use
water, 70% EtOH or isopropanol, if needed.
Note
: Do not use aggressive solvents such as strong acids or bases or organic solvents at
any time (see also page 41 solvent compatibility). If unsure please
contact
for detailed information about your specific reagent/solvent.
8. It is possible to enter a sample name for each sample in the input window “enter sample
name”.
Note
: Allowed characters are: A…Z a…z 0…9 , . - ( ) @ ! = _ ~ ; [ ] { } ‘
Note:
Blank character is not allowed.
9. Mix the sample very well to achieve a homogenous sample.
10. Apply the appropriate amount of sample solution onto the measurement window and initiate
the sample measurement with the sample button. Upon completion of measurement remove
the lid, clean the surfaces and apply the next sample.
S
AMPLE
H
ANDLING
T
IPS
Nanovolume Methods
•
The NanoPhotometer
®
includes an integrated vortexer (N60/NP80 only) to assure sample
homogeneity. It is recommended to vortex every sample right before the measurement.
•
The sample window is illuminated (NP80/N60/N50 only) with a low energy red light to assist
with accurate sample application. The red light is switched off once the sample arm is
closed. It is possible to disable the illumination feature in preferences of the NPOS software.
•
The minimum volume that can be used for nanovolume samples is 0.3 µl (NP80/N60/N50
dsDNA > 420 ng/µl and BSA > 12.6 mg/ml and for submicroliter cell in the C40 dsDNA > 25
ng/µl and BSA > 0.7 mg/ml). For automatic path length setting at least 1µl is needed
(NP80/N60/N50 only).
•
The maximum volume that can be used for nanovolume samples is 2.0 µl (NP80/N60/N50)
and 5 µl for the submicroliter cell with lid 5.
•
The sample can be fully recovered after measurement with a pipette if desired.
Note:
Minimal cross contamination cannot be avoided on a molecular level.
•
Proper cleaning is important to ensure accurate measurements. In most cases a dry lint-free
laboratory wipe is sufficient to clean the sample quartz surfaces. In the case of highly
concentrated samples or certain proteins, the recommended procedure for cleaning is to
