NanoEnTek JuLi Stage, User Manual

Page: 139 / 150

JuLI™ Stage STAT Software
JuLI™ Stage - User Manual
139
For example, a 96 well plate was selected:
There are 4 tabs available to allocate and describe detailed conditions for each well
of a plate:
Compounds, Cells, Conditions or Regions . (Headers of these tabs
cannot be changed.)

Compounds : Name, concentration and dilution series of compounds can be
entered and assigned to wells.

Cells : Cell types, passage number, seeding density and dilution series can be
entered to wells.

Conditions : Can be used to assign any other important information to wells.

Regions : Wells can be bundled to regions, which will ease the selection of wells
in the other tabs.
The first 2 tabs offer additional sub-menus in separate windows to support and ease
filling in concentrations or cell numbers, however, there are two important facts to
consider:

Firstly, all entries of all tabs will appear in the header of result tables and on the
legends of graphs.

And secondly, concentrations of compounds are neither selectable on the x-axis
of graphs nor can they be exported and accessed in secondary analyses such
as IC
50
calculations.
All plate map entries are only aiming at

localizing well repeats in order to calculate mean values and standard deviations
or

the detailed description of conditions of certain wells.
☞
Note: In order to allocate well-repeats in a plate layout (e.g. to calculate mean
values of triplicates) it is not necessary to fill in all 4 tabs, one tab (compounds, cells
or conditions) is already sufficient.