JuLI™ Stage STAT Software
104
JuLI
TM
Stage - User Manual
measurement project in
JuLI
TM
EDIT
and save the original measurement under a
new name. For more details, see chapter 11
“JuLI™ EDIT Software”
☞
Note: Parameters to optimize confluence segmentation on fluorescent images
(
Fluorescent Param
) are deactivated for the brightfield channel.
☞
Note: How to analyze the whole measurement project, create graphs and export
result tables once all settings are optimal, see later in this chapter.
Optimizing confluence analysis on fluorescent images
In order to analyze the percentage of confluence on fluorescence images, first a
fluorescence channel needs to be selected by clicking it.
There are many settings available to optimize the confluence analysis:
Background
Control
,
Default Gating
,
Tolerance Factor
,
Maximum intensity zero
, manually
gating min and max intensities,
Quick Mode
and the optimization of
Brightness
and
Contrast
on the images in
Edit Image
.
Start to optimize the confluence analysis by selecting a representative well, time
point and field in a well and then click
Apply
using the default settings shown above.
Summary of Contents for JuLi Stage
Page 1: ......