NanoEnTek JuLi Stage, User Manual

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Operation
JuLI™ Stage - User Manual
45
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Note: In case both manual focus and auto focus are activated, the Z-height of the
manual focus will be used where applied, all other positions will be focused using
the auto focus.
Auto Focus : You can choose how many steps to make until the next well that is
supposed to be focused using the auto focus (1 = next well, 2 = second next well).
Setting auto focus to 4 will image well 1, 5, and 9 and so on as shown below by the
blue or grey wells.
All Positions : Similarly to the Auto Focus you can choose how many steps to make
until the next position that is supposed to be focused using the auto focus (1 = next
position, 2 = second next position). Setting All Positions to 4 will image position 1,
5, 9, and 13 as shown below by the light blue squares.
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Note: In case All Positions is turned off, auto focus will be activated in the first
position of the well only.
All Channel : In order to focus on each channel individually All Channel can be
used. If inactive, only brightfield will be used for the auto focus.
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Note: When All Channel is turned on, Custom AF is not activated.
Every Cycle AF : Activate Every Cycle AF to use the auto focus in every cycle.
This helps maintaining the correct focus, however, increases photobleaching/
phototoxicity.