JuLI™ Stage STAT Software
132
JuLI
TM
Stage - User Manual
Intensity
gating: Minimum intensity
Set
to 20, maximum intensity set to 100, then
Apply
In the
Cell Size
tab, additional options to influence segmentation are available:
thresholds to exclude certain cell sizes can be set. There is a mouse hover in image
view which displays the cell size of the cell pointed at to support this decision
process, next to a histogram display (x-axis: size in [µm²], y-axis: number of objects).
Cell Size
set minimum and maximum, apply new value by clicking
Set
and
Apply
(In the example below, no gating value was applied).
☞
Note: Finding the best combination of settings is an iterative process and should
always be tested on representative images (different time points, positive and
negative controls, etc.).
The remaining functions of the
Attached Cell Counting
module (
Analyze
,
View
Results
) are identical to
Growth Curve
and described in chapter 0
” in detail.
☞
Note: If more than one position was acquired in a well, the readout [n/well] is
equal to the sum of all cells counted in all positions in a well. For example: 3 positions
were acquired with a 10x objective (0.58 mm² per FOV) in a 384 well plate. 180, 126
and 85 cells/image were counted. Then the mean would be 130 cells/image, and
based on the mean there are 224 cells/mm². The sum would be 391 cells/well
(=180+126+85).
Summary of Contents for JuLi Stage
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