NanoEnTek JuLi Stage, User Manual, Page: 44 / 150

Operation
44 JuLI
TM
Stage - User Manual
The stack can be acquired downwards, upwards, or up- and downwards from the
current position.
☞
Note: Stitching and Z-stack acquisition are mutually exclusive.
Manual Focus
Using Manual Focus images will be acquired in a Z height defined by the user.
1. Activate the manual focus.
2. Focus on your object manually or using the auto focus and click Set Focus
for each channel in the focus adjustment area below the image view.
3. Repeat this for all relevant positions.
4. Review all positions clicking Focus Info (see below)

A1: Only for brightfield a manual focus has been set.

A2: Brightfield and GFP have a set focus.

A3: Brightfield, GFP and RFP have a set focus.
Auto Focus
The image based auto focus is highly flexible and can be modified to focus in various
different configurations. These options ensure that the user can save time, lower
phototoxicity, correct for uneven plate bottoms, or moving samples while
maintaining the optimal focus.