Operation
JuLI™ Stage - User Manual
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7.5 Quick Workflow (setting up an experiment)
1. Before the experiment determine the optimal objective and exchange the
objective if necessary. See chapter 7.4
“ Settings menu” (How to change the
objective).
2. Place the sample into the plate holder of the XY-stage
3. Open the control software and go to the
Setup
menu
4. Enter an appropriate experiment name
5. Turn on the brightfield mode and focus on the well border (some lids of round
bottom plates have condensation rigs which can be used to calibrate plates as
well).
6. Click
Calibration
, select a plate type and calibrate it. See chapter 7.1
“ Preview menu” (Vessel type).
7. In the plate layout select all wells that are supposed to be imaged and click
Add
Position
to add the center field of all selected wells to the position list.
Alternatively select all desired wells, then add positions to the currently shown
well by clicking into the well navigation and click
Add Position
to add all
positions to all wells.
8. Select a representative well and set up your channel settings below the image.
All channels that are supposed to be captured have to be selected below the
plate layout. See chapter 7.1
“ Preview menu” (Channel selection).
9. Activate stitching or z-stack, or the scratch-mode (if available). Stitching and z-
stacks are applied to all positions
10. Choose the image output
11. Set the time lapse settings
12. Switch on
Save Protocol
if you want to re-use these settings
13. Start and review the time lapse settings
14. Confirm the experiment a second time by clicking
Play
.
Summary of Contents for JuLi Stage
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