Operation
JuLI™ Stage - User Manual
35
1. Click
Guide Line
to get an idea where exactly the center of the image is.
2. Navigate the guide lines onto the position and click
Add
to set a mark and
assign a name or description to the position.
Channel selection
1. By clicking one of the channel buttons the corresponding LED is activated.
2. To turn off the LED please click the same channel button again or simply
activate another channel.
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Note: If not deactivated the LED will remain active. To reduce
photobleaching/phototoxicity please minimize illumination time by deactivating
the LED whenever possible.
Acquisition settings
The acquisition settings allow the user to adjust the exposure time, brightness
(digital intensity gain), and the power of the LED. D-Phase increases the
stereoscopic features of an image, giving it a stronger depth and thus slightly
enhances edges. However, it can also create slight background noise.
Exposure range : 5 ms - 500 ms
Brightness range : 0 - 40
LED power range : 1 - 10
D-Phase range: 0 - 10
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Note: When setting long exposure times, high LED power and high brightness for
the GFP channel, a brightfield-like background image may appear. Try to avoid this
by using bright GFP-samples or low to medium GFP exposure, brightness, and
LED-power settings.
Auto focus
To use the image based auto focus click
Auto Focus
.
Summary of Contents for JuLi Stage
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