NanoEnTek JuLi Stage, User Manual

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JuLI™ Stage STAT Software
90 JuLI
TM
Stage - User Manual
12
JuLI ™ Stage STAT Software
12.1 Software overview
With JuLI
TM
Stage STAT, projects containing measurement data are selected and
analyzed with one of four evaluations: Growth Curve , Scratch Basic , Attached
Cell Counting and Whole Intensity Level .
With Plate Editor, detailed experiment conditions of the selected project can be
defined and saved in plate maps: each well can be assigned to specific assay
conditions (such as cell type and number, compound concentration etc.) in order to
calculate e.g. mean values of triplicates and their standard deviations.
In addition, the View Results section of each evaluation module also offers to create
or load a saved experiment layout.
All four evaluation modules lead to tables and ready-to-use graphs which can be
copied and pasted. Numerical analysis results are presented in an exportable .csv
format for further processing with external software such as Microsoft Excel
®
or other
spread sheet software.
Evaluation
module Input
channel Output metrics Example assays
Growth
Curve
BRIGHT
GFP,
RFP,
DAPI Confluence = Growth rate
[%] or [µm²/image],
[µm²/well] [mm²/image]
[mm²/well]
Mean fluorescence intensity
per well [-] (only for
fluorescence channels) Cell health and
viability assays,
cytotoxicity,
transfection rate,
cell culture quality
control
Scratch
Basic BRIGHT
GFP,
RFP,
DAPI Confluence in wound =
Growth rate (in wound) [%]
or [µm²/image], [µm²/well]
[mm²/image] [mm²/well]
Mean fluorescence intensity
per well [-] (only for
fluorescence channels) Scratch wound
assay, migration
and invasion,
different
magnifications
Attached
Cell
Counting BRIGHT
Counting result [n/image],
[n/mm²] and [n/well],
n=number of adherent cells
Cell health and
viability assays,
cytotoxicity,
transfection rate