Life technologies invitrogen Neon, User Manual

Page: 39 / 52

Troubleshooting
Problem Cause Solution
No power (the
display remains
blank when the
power is turned
on) AC power cord is not
connected Check AC power cord connections at both ends. Use the correct
cords.
Pipette or tube is
incorrectly inserted

Properly insert the Neon
™
Pipette into the Neon
™
Pipette
Station as described on page 20. The metal head of the
pipette should be tightly connected to the ball plunger
inside the pipette station.

Properly insert the Neon
™
Tube into the Neon
™
Pipette
Station as described on page 15. The side electrode on the
tube should be tightly connected to the ball plunger inside
the pipette station.

Avoid spilling any liquid into the pipette station to prevent
any build up of rust on the ball plunger in the pipette
station.
Connection error
message
displayed
The sensor connector
is not connected

Be sure to connect the sensor connector of the Neon
™
Pipette
Station to the sensor port on the rear of the Neon ™ device.

Make sure the mark on the cable plug and the instrument
connector is aligned correctly (page 6)
Error messages — See page 33 for a description of error messages.
No Neon
™
Tip is
inserted or the Neon
™
Tip is inserted
incorrectly
Make sure that the Neon
™
Tip is inserted into Neon
™
Pipette
correctly as described on page 20. There should be no gap
between the tip and the top head of the pipette.
No buffer in the tube
or no sample in the
tip Be sure to add 3 mL of the appropriate Electrolytic Buffer to
Neon
™
Tube. The electrode in the tube must be completely
immersed in buffer.
Be sure to add sample in Resuspension Buffer to the Neon
™ Tip.
Wrong buffers used Use the Electrolytic Buffer (Buffer E for 10 μL tip and Buffer E2
for 100 μL tip) in the Neon
™ Tube and the sample in
Resuspension Buffer in the Neon
™
Tip. Do not switch buffers or
use any other buffer as these buffers are specifically designed
for electroporation with the Neon
™
device.
Connection
failure
High voltage
connector is not
connected Be sure to connect the high voltage connector of the Neon
™
Pipette Station to the high voltage port on the rear of the Neon
™
device (page 6).
If the error
persists and all
connections are
correct Perform self
diagnostics test Perform self diagnostics test by clicking on

on the main
screen. During the self diagnostics test, the device checks a
variety of parameters and indicates if it is OK or there is a
problem. If the self diagnostics is OK, ensure that all connections
are correct as described in this section before contacting
Technical Support (page 39).
Continued on next page
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