Getting Started,
Continued
Installing the
Neon
™
Device with
Pipette Station,
Continued
8.
Attach the power cord to the AC inlet on the rear of the Neon
™
device and
then to the electrical outlet. Use only properly grounded AC outlets and power
cords.
9.
To turn on the power, press the main power switch on the rear of the unit to
ON
position. The digital display shows start up screen (next page).
10.
The Neon
™
device is operated by the touch screen on the front of the device.
You can easily input electroporation parameters by lightly touching the touch
screen with a finger tip or a touch screen pen. See next page for details.
You are ready to use the Neon
™
Transfection System. See page 14 for details.
Registering the
Device
Visit
www.invitrogen.com/neon
to register the device and activate your
warranty or extended warranty, and ensure that you receive product updates,
special offers, and faster service.
Electroporation
Protocol Options
There are three options to select an electroporation protocol for your cell type:
If you already have the electroporation parameters for your cell type, input
the parameters in the
Input Window
(see below).
If you wish to add cell-specific electroporation parameters to the database
on the device for future use, input the parameters in the
Database Window
(page 9). You can also view our library of protocols for commonly used cell
types from www.invitrogen.com/neon and in put the parameters in the
Database Window (see below) for various cell types.
If you do not have any specific electroporation parameters for your cell type
and wish to perform optimization, use the
Optimization Window
(page 11).
Input Values Limit
The Neon
™
device is designed to only input certain values and limits for each
value are listed below. If your input value exceeds the maximum value, an
error is displayed.
Input Voltage range: 500–2,500 V
Input Pulse Width range: 1–100 ms
Input Pulse Number range: 1–10
Continued on next page
7
