Optimization Protocol for DNA and siRNA,
Continued
Choosing
Appropriate
Optimization
Protocol
Based on your cell type, choose the appropriate optimization protocol as shown
below. Optimizations are generally required for cell types which are not in the
Neon
™
database but may also be needed for cell types that exist in the Neon
™
database as cell culture conditions may vary between laboratories.
Cell Type
Cell Line
Primary Cells
Adherent
Suspension
Suspension
Adherent
Resuspension
Buffer R
Resuspension
Buffer T
Resuspension
Buffer R
Resuspension
Buffer R
Examples include:
3T3-L1, HEK293,
COS-7, C2C12,
HeLa, HCT-15,
PC-12, MDCK,
Raw264.7, U-2OS
Examples include:
T-cells, B-cells,
NK cells, PBMC,
Monocytes
Examples include:
Neuronal, Stem,
Hepatocyte,
HUVEC,
Macrophage,
Dendritic
Examples include:
CEM, HL-60,
K-562, Jurkat,
LCL, Ramos,
U-937
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