Using the Neon
™
Transfection System,
Continued
Electroporation
Protocol,
Continued
8.
Insert the top-head of the Neon
™
Pipette into the Neon
™
Tip until the clamp
fully picks up the mounting stem of the piston (see below)
Top-head
9.
Gently release the push-button, continuing to apply a downward pressure on
the pipette, ensuring that the tip is sealed onto the pipette without any gaps.
Note:
Ensure that the Neon Pipette and Tip are tightly connected without a gap (see
figure on the left) for trouble-free pipetting and proper electrical connection.
O
ⅹ
10.
Press the push-button on the Neon
™
Pipette to the first stop and immerse the
Neon
™
Tip into the cell-DNA/siRNA mixture. Slowly release the push-button
on the pipette to aspirate the cell-DNA/siRNA mixture into the Neon
™
Tip.
Note:
Avoid air bubbles during pipetting as air bubbles cause arcing during
electroporation leading to lowered or failed transfection. If you notice air bubbles in the
tip, discard the sample and carefully aspirate the fresh sample into the tip again without
any air bubbles.
air bubbles
Continued on next page
19
