About the Product,
Continued
System Overview
Unlike standard cuvette based electroporation, the Neon
™
Transfection System
uses a unique electroporation reaction chamber, the Neon
™
Tip that delivers a
high electric field to the biological sample. The Neon
™
Tip maximizes the gap
size between the two electrodes while minimizing the surface area of each
electrode. As a result, the sample experiences a more uniform electric field,
minimal pH change, less ion formation, and negligible heat generation.
This next generation electroporation technology overcomes many of the
limitations associated with standard cuvette based electroporation thereby
increasing transfection efficiency and cell viability, and providing an ergonomic
workflow.
V
V
V
The transfection occurs in the uniquely designed Neon
™
Tip using simple 3-
step procedure.
1.
Load a mixture of harvested cells and molecules to be delivered (e.g., DNA,
RNA, siRNA) into the Neon
™
Tip.
2.
Plug the Neon
™
Pipette with Neon
™
Tip into position in the Neon
™
Pipette
Station with Neon
™
Tube; select your protocol on the device, and press
Start
.
3.
Unplug the Neon
™
Pipette and transfer your transfected cells into a tissue
culture vessel containing the appropriate medium.
Features
Important features of the Neon
™
Transfection System are listed below:
User-friendly Neon
™
device benchtop design that easily fits in your tissue
culture hood for easy, efficient transfection of a wide variety of
mammalian cells including primary and stem cells
Ability to transfect 1 × 10
4
–5 × 10
6
cells per reaction in a sample volume of
10 μL or 100 μL in a variety of cell culture formats (60 mm, 6-well, 48-well,
and 24-well)
Utilizes a single buffer system for all cell types except primary suspension
blood cells
Simple touch screen interface for easy programming of electroporation
parameters
Available with one pre-programmed 24-well optimization protocol and
the option to customize up to 50 cell specific protocols
Built-in safety features in the device to enhance user safety
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