Chapter III. The BAX® System X5 PCR Assays
BAX® System X5 User Guide
51
Reviewing Results Graphs Well Chart
Target peaks
–
Positive
reactions show target peaks within a specified temperature range. Peaks can shift within
their temperature range. In strong positive reactions, the peaks can shift to slightly lower temperatures, and in weaker
reactions, the positives can shift to higher temperatures.
Control (INPC) peaks
–
INPC peaks occur in a specific temperature range for each target. Peaks vary in height
depending on the presence or absence of the target. At high target levels, the control peak may be very small or
absent.
Strength of positive result
–
The
strength of a positive result is determined by comparing the peak heights of the
target to the control. Samples with high target levels will display a strong positive result as a tall target peak and
shorter control peak. In reactions with lower target levels, the control peak grows taller as the target peak gets
shorter. When the target peak displays at less than 1/10 the height of the control peak, the positive result is
considered weak.
