Chapter III. The BAX® System X5 PCR Assays
BAX® System X5 User Guide
49
Negative Salmonella Melting Curve Profile
Samples that are negative for
Salmonella
will display no target peaks. The graph displays only the INPC, or control
peak, which usually appears between 76-80°C.
Example of Typical Melt Curve for Negative Salmonella Sample
7. Confirm Positive Results
Method approved by AOAC
Positive results can be confirmed according to the AOAC confirmation method by following the reference culture
method appropriate for the sample type. To confirm results with the FDA-BAM method, see the protocol described in
the Bacteriological Analytical Manual. To confirm results with the USDA-FSIS method, see the protocol described in
the Microbiology Laboratory Guidebook.
