Chapter III. The BAX® System X5 PCR Assays
BAX® System X5 User Guide
41
6. Review Results
Note: For more information about reading BAX® System results, see
“
Reviewing BAX® System Results
”.
When processing in the BAX® System X5 instrument is complete, a new window appears showing each color-cued
well with an icon in the center to illustrate the result:
Green (-)
=
Negative result
Yellow (?)
=
Indeterminate
result
Red (+)
=
Positive result
Yellow (?) with red slash
=
Signal error
Positive L. monocytogenes Melting Curve Profiles
Samples that are positive for
L. monocytogenes
will display one target peak. This peak usually appears between 83-
88°C.
The graph also displays one INPC, or control peak, which usually appears between 76-81°C. Often, the control peak
appears as a
“double-peak” in the presence of
L. monocytogenes
. If a sample displays a strong positive result, the
INPC may be very small in comparison to the target peak.
Examples of Typical Melt Curves for Positive L. monocytogenes Samples
Negative L. monocytogenes Melting Curve Profile
Samples that are negative for
L. monocytogenes
will display no target peaks. The graph displays only the INPC, or
control peak, which usually appears between 76-81°C.
Example of Typical Melt Curve for Negative L. monocytogenes Sample
