Hygiena BAX System X5, User Manual

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CONTENTS
Introduction and Overview ...................................................................................................................................... 1
About the BAX® System ......................................................................................................................................... 1
User Assumptions ................................................................................................................................................... 2
Text Conventions .................................................................................................................................................... 2
Ordering Information ............................................................................................................................................... 3
Other Required Equipment and Supplies ................................................................................................................ 4
Storage and Disposal Information ........................................................................................................................... 5
Shipping Instructions ............................................................................................................................................... 6
Technical Assistance .............................................................................................................................................. 6
I. The BAX® System X5 Instrument ........................................................................................................................ 7
Instrument Specifications ........................................................................................................................................ 7
Environmental Parameters...................................................................................................................................... 7
Instrument Components .......................................................................................................................................... 8
Safety Precautions .................................................................................................................................................. 9
Instrument Installation ........................................................................................................................................... 11
Instrument Configuration ....................................................................................................................................... 11
Instrument Maintenance ....................................................................................................................................... 13
Instrument Decontamination ................................................................................................................................. 13
Capping/Decapping Tools ..................................................................................................................................... 15
II. The BAX® System X5 Software........................................................................................................................ 17
The BAX® System X5 Software Window .............................................................................................................. 17
Creating a Rack File ............................................................................................................................................. 19
Printing Reports .................................................................................................................................................... 20
Installing and Upgrading the BAX® System Software ........................................................................................... 22
III. The BAX® System X5 PCR Assays ................................................................................................................. 24
BAX® System Assay, Assay Validation ................................................................................................................ 24
Using the E. coli O157:H7 Assay ........................................................................................................................ 255
Using the Genus Listeria Assay .......................................................................................................................... 311
Using the L. monocytogenes Assay .................................................................................................................... 377
Using the Salmonella Assay ............................................................................................................................... 433
Reviewing BAX® System Results ......................................................................................................................... 50
Best Practices for Sample Enrichment and Preparation………………………………………………………………..52
Using Analog Heating and Cooling Blocks with the BAX® System Assays ........................................................ 524
IV. Troubleshooting the BAX® System ............................................................................................................... 555
Accessing Log Files ............................................................................................................................................ 555