BAX® System X5 User Guide
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III. The BAX® System X5 PCR Assays
Each BAX® System PCR assay includes everything required for preparing sample enrichments, including lysis buffer
and protease for sample preparation and strips of PCR tubes containing BAX® System PCR tablets for automated
processing in the BAX® System instrument. These PCR tablets combine all the requisite reaction reagents, including
fluorescent dye and an internal positive control, into a stable, dry tablet for simplicity and convenience - simply
hydrate them with prepared sample and load the rack into the BAX® System instrument.
Assay Validation
The protocols in this chapter have been validated by Hygiena for use with the BAX® System assays. In order to
follow the BAX® System method, the enrichment and sample preparation protocols must be followed exactly as
described in this user guide and the kit instructions.
For many sample types, standard enrichment protocols (such as the USDA-FSIS, FDA-BAM or ISO methods) may
also be used. These enrichment protocols should be internally validated before use with the BAX® System, as these
methods have not been validated by Hygiena or any third-party organizations. To test a sample type that is not listed,
please contact Hygiena Diagnostics Support for recommendations on enrichment and sample preparation.
