Chapter III. The BAX® System X5 PCR Assays
44
BAX® System X5 User Guide
Custard, 2% milk,
chilled ready meal,
cooked fish, prawns,
macaroni, pizza
dough, frozen peas,
orange juice, cream
cheese, and dry pet
food
Homogenize 25 g sample with 225 mL pre-warmed
(35°C) LB. Let stand at room temperature for 55-65
minutes. Incubate at 35°C for 22-26 hours.
Transfer
10 μL enriched
sample to 500 μL pre-
warmed (37°C) BHI.
Incubate at 37°C for 3 hours.
Liquid eggs
Homogenize 25 g sample with 225 mL pre-warmed
(35°C) BPW. Incubate at 35°C for 22-26 hours.
Transfer 10 µL enriched
sample to 500 µL pre-
warmed (37°C) BHI.
Incubate at 37°C for 3 hours.
Soy protein flour
Pour 25 g sample slowly over 225 LB in a flask. Let
stand for 55-65 minutes at room temperature. Cap
loosely. Incubate at 37°C for 18-22 hours.
Transfer 10 uL enriched
sample to 500 uL pre-
warmed (37°C) BHI.
Incubate at 37°C for 3 hours.
1. Enrich Samples (continued)
Sample type
Primary enrichment
Secondary enrichment
Alfalfa sprouts
Homogenize 25 g sample with 225 mL pre-warmed
(42°C) BPW with 20 mg/L novobiocin. Incubate at 42°C
for 20-24 hours.
Transfer 10 µL enriched
sample to 500 µL pre-
warmed (37°C) BHI. Incubate
at 37°C for 3 hours.
Stainless steel,
ceramic tile, plastic
and epoxy coated
tile
Homogenize sponge with 225 mL pre-warmed (35°C)
LB. Let stand for 55-65 minutes at room temperature.
Incubate at 35°C for 22-26 hours.
Transfer 10 µL enriched
sample to 500 µL pre-
warmed (37°C) BHI. Incubate
at 37°C for 3 hours.
The
French Association of Normalization (AFNOR Certification)
has
certified the BAX® System according to the NF VALIDATION rules for detecting
Salmonella in all human food products, animal feed and environmental samples
(except primary production stage environmental samples).
Sample type
Primary enrichment
Secondary enrichment
Standard media
Raw meats and
poultry (without
spices)
Homogenize 25 g sample with 225 mL room
temperature BPW. Incubate at 37°C for 16-20 hours.
None
Dairy (except
powdered milk)
Homogenize 25 g sample with 225 mL room
temperature BPW supplemented with 20 mg/L
novobiocin. Incubate at 42°C for 20-24 hours.
None
Other foods and
environmental
samples
Homogenize 25 g sample with 225 mL room
temperature BPW. Incubate at 37C for 16-20 hours.
Transfer 10 µL enriched
sample to 500 µL room
temperature BHI.
Incubate at 37°C for 3-4
hours.
