Chapter III. The BAX® System X5 PCR Assays
48
BAX® System X5 User Guide
6. Review Results
Note: For more information about reading BAX® System results, see
“Reviewing BAX® System Results”.
When processing in the BAX® System X5 instrument is complete, a new window appears showing each color-cued
well with an icon in the center to illustrate the result:
Green (-)
=
Negative result
Yellow (?)
=
Indeterminate
result
Red (+)
=
Positive result
Yellow (?) with red slash
=
Signal error
Positive Salmonella Melting Curve Profiles
Samples that are positive for
Salmonella
will display three target peaks. These target peaks usually appear between
82-91°C, with approximately 5°C distance between the first and the third target peak. If a sample displays a strong
positive result, the target peaks may merge so that you see only two distinct peaks.
The graph also displays one INPC, or control peak, which usually appears between 76-80°C. If a sample displays a
strong positive result, the INPC may be very small in comparison to the target peaks.
Examples of Typical Melt Curves for Positive Salmonella Samples
