horiba OCMA-500, Instruction Manual, Page: 70 / 141

Measurement
50

Extraction

Items required

Separating funnel (300 mL)
*1
: 2 (for sample extraction and for zero point checking)

Measuring cylinder (200 mL)
*1
: 3 (for sample, for solvent, and for clean water)

Dropper (provided): 1 (for hydrochloric acid)

pH meter

Filter paper (if suspended material will be removed and as needed)
*2

Glass container (if suspended material will be removed or water will be removed from
solvent layer)
*1

Teflon membrane filter (mesh diameter 20

m to 40

m) (if water will be removed from
solvent layer)

Glass funnel
*1
(if water will be removed from solvent layer)

Measuring utensil (if water will be removed from solvent layer)

Sample water

Clean water
*3

Hydrochloric acid (refer to "Hydrochloric acid" (page 22))

Clean solvent S-316 (optional)

Anhydrous sodium sulfate (Na
2
SO
4
) (if water will be removed from solvent layer)
Note
*1: Clean the glass utensils with solvent in advance and let air dry.
*2: Use solvent to elute and clean organic material from the filter paper in advance, and let air dry.
*3: Use the same clean water for the sequence of tasks from zero calibration to measurement.

Normally pure water should be used.

If the sample contains large amounts of water-soluble substances, such as urea or NaCl, and the
concentration is known, perform zero calibration using this oil-free aqueous solution.

Extraction procedure
1.
Measure 200 mL of clean water into the measuring cylinder for clean water,
measure 100 mL of clean solvent into the measuring cylinder for solvent, and
dispense these into the separating funnel for zero point checking.
2.
Measure 160 mL to 200 mL of sample water into the measuring cylinder for sample,
and dispense into the separating funnel for sample extraction.
Tip
If the sample contains emulsifying substances, it is recommended that you use a smaller amount of
sample water (approx. 100 mL).
3.
Measure an amount of clean solvent equal to 1/2 the amount of sample water in the
measuring cylinder for solvent, and transfer this to the measuring cylinder for
sample.
4.
Rinse the inside of the measuring cylinder for sample with the solvent, and add this
solvent to the separating funnel for sample extraction.
5.
Use the dropper to add 0.2 mL to 0.5 mL (5 drops to 10 drops) of hydrochloric acid
to each separating funnel (for sample extraction and for zero point checking).
Tip
If the sample contains emulsifying substances, there is no need to add hydrochloric acid.