AP Blot– SERVICE MANUAL
SECTION 4
CALIBRATION PROCEDURES (XCALIB)
Doc. N° MDS-AP-26-00-02
Rev.01
Date: 02.04.2009
Pag. 89
4.8.2. Calibrate probe height
From “
Dilutor
” pull down menu select “
Calibrate probe height
”.
Height Calibration determines the safe distance between the probe tips and the bottom of
the wells in each section of the Work Area– this the number of maximum steps that a
probe can go down (Z maximum height)
The following display appears:
Calibration
The number of steps - Z maximum height - should be calculated for each section of the Work Area:
Sample and reagent section
1. Insert an empty test-tube in position 1 of sample rack
2. Insert empty bottle in reagents rack in following positions: R1 (dilutor area), R2 (reagent area), in CL
(cleaning solution area) and C1 (control area)
3. By selecting sample rack in Work Area, position 1 and the last stored maximum height value are also
automatically selected
4. Select “
Go down
” command. The probe will go down the number of steps shown in the “
Height
” box.
5. Now check if probe is:
If probe is touching bottom of well
: type in 100 less steps from the current value displayed in “
Height
” box
Repeat procedure from point (4)
If probe is NOT touching bottom of well
: select the “
Increase
” arrow and probe will automatically return to
home position and then go down again with an additional 10 steps.
Repeat procedure until probe touches bottom of well
Select “
Store
”. 10 steps will be automatically subtracted from previous height and new value will be stored
(e.g.: 1690 => 1680).
6. To return to Work Area select “
Work Area”
key
7. Select reagent area and repeat procedure for positions R1 (dilutor area), R2 (reagents) CL (cleaning
solution) and C1 (control area)
