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AP Blot– SERVICE MANUAL

SECTION 4

CALIBRATION PROCEDURES (XCALIB)

Doc. N° MDS-AP-26-00-02
Rev.01
Date: 02.04.2009

Pag. 84

4.8. DILUTOR

4.8.1. Calibrate probe positions

From “

Dilutor

” menu select “

Calibrate probe positions

”.

The following display will appear:

Any part of the Work Area can be selected with a mouse click on it. The probes will automatically move into the first
position over the selected area. Different commands of panel are activated/disactivated depending on which section
of the Work Area is selected.

Work Area

Command Panel

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Summary of Contents for AP-BLOT

Page 1: ... 637070 Fax 0774 634039 E mail info dasitaly com http www dasitaly com AP AP AP AP BLOT BLOT BLOT BLOT SERVICE MANUAL SERVICE MANUAL SERVICE MANUAL SERVICE MANUAL This manual contains DAS reserved information All rights reserved Unauthorized copying of this manual or parts is prohibited ...

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Page 3: ...Verifica Approval Approvazione 0 Rev 00 29 04 08 Issue 0 Rev 01 02 04 09 General revision Important Notes To avoid errors during maintenance and repairs carefully read the entire manual before attempting any work on the instrument Pay particular attention to the GENERAL SAFETY WARNINGS in Section 1 and follow instructions for specific operations precisely as indicated in this manual ...

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Page 5: ...ve mechanism 32 3 3 3 5 Substituting Peristaltic Pump Tubing 33 3 3 3 6 Substituting Dilutor Motor 34 3 3 3 7 Substituting Opto SIDILOP PCB 35 3 3 3 8 Substituting INTERFA2 PCB 35 3 3 3 9 Substituting probe wash basin 35 3 4 X Y AXIS 36 3 4 1 DESCRIPTION OF HOW IT WORKS 36 3 4 1 1 X Y axis movement mechanism 36 3 4 1 2 X Y axis Electrical Functions Diagram 37 3 4 2 REGULAR MAINTENANCE FOR X Y AXIS...

Page 6: ...3 8 2 1 Cleaning opto 56 3 8 2 2 Checking procedure 56 3 8 3 CHECKING DISTANCE FOR OPTO COUPLERS X AXIS 57 3 8 4 CHECKING DISTANCE FOR OPTO COUPLERS Y AXIS 58 3 9 USB COMMUNICATION 59 3 9 1 DESCRIPTION OF HOW IT WORKS 59 3 9 2 CHECK FUNCTIONALITY 59 3 9 3 REPLACING DILAP21 and SIR4MOT PCBs 60 3 10 DILUTOR COMMAND LIST 61 3 11 HYDRAULIC CIRCUIT DIAGRAM 66 3 11 1 REGULAR MAINTENANCE 67 3 11 2 SUBSTI...

Page 7: ...93 4 8 5 Calibrate syringe 95 4 8 6 Calibrate SUL 98 4 8 7 Wash basin test 99 4 8 8 Prime 101 4 8 9 Calibrate camera 102 4 8 10 Calibrate parameters 104 4 8 11 Home 105 4 8 12 Turning motors off 105 4 8 13 Reset 105 4 9 106 4 9 1 Instrument information 106 4 9 2 Software component information 107 4 9 3 About 107 SECTION 5 TROUBLE SHOOTING 109 5 1 DILUTION DISPENSATION 111 5 2 X Y Z MOVEMENTS 112 5...

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Page 9: ...AP Blot SERVICE MANUAL SECTION 1 GENERAL SAFETY Doc N MDS AP 26 00 02 Rev 01 Date 02 04 2009 Pag 9 SECTION 1 GENERAL SAFETY ...

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Page 11: ...ion of the document should be read carefully 1 2 INSTRUMENT USE AND CONDITIONS The instrument is intended for use in the following working conditions as In Vitro Diagnostic IVD medical device as specified in the technical data with chemical reagents and accessories supplied and or declared compatible with instrument at a specific temperature and humidity levels as specified in this manual not to b...

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Page 13: ...AP Blot SERVICE MANUAL SECTION 2 INSTALLATION AND TECHNICAL SPECIFICATIONS Doc N MDS AP 26 00 02 Rev 01 Date 02 04 2009 Pag 13 SECTION 2 INSTALLATION AND TECHNICAL SPECIFICATIONS ...

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Page 15: ...n both sides 3 4 for manual instrument placement Once in position remove the handles e put back in place the plastic covers b Remove the 4 screws that hold the instrument on to the wood base 5 6 c Remove screw and bracket 7 screw in the same place using the screw removed before d Remove screw and bracket 7 screw in the same place the enclosed screw in the bag e Remove screws 8 9 10 and related bra...

Page 16: ...rocess up to strips on oscillating plane Dispensation System 1 dispensation and 1 aspiration probe Strip Washing Liquid dispensation and aspiration via two instrument probes Image acquisition system High Resolution Colour Camera 3 3 Megapixels mounted above Probes Dispensation Up to 2500 µl with a 1 µl resolution Software Windows 2000 and XP compatible to set up work list reports and result archiv...

Page 17: ...AP Blot SERVICE MANUAL SECTION 3 MAINTENANCE PROCEDURES Doc N MDS AP 26 00 02 Rev 01 Date 02 04 2009 Page 17 SECTION 3 MAINTENANCE PROCEDURES ...

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Page 19: ...l unit Description X Y Axis The X Y axis movement mechanism moves probe arms over work area with precision Z1 Axis The Z1 axis raises and lowers dispensing probe over work area Z2 Axis The Z2 axis raises and lowers aspiration probe over work area Dilutor unit The dilutor unit aspirates and dispenses samples calibrators controls and generally all reagents It also washes Z1 axis probe internally and...

Page 20: ...URES Doc N MDS AP 26 00 02 Rev 01 Date 02 04 2009 Page 20 The AP Blot communicates with the PC via a serial connection between the Dilutor PCB and the PC The units controlled by the PCB are PCB Unit X Y Axis Z1 Z2 Axis Dilutor unit DILAP21 Shaking plane ...

Page 21: ... 2 1 REMOVING WORK AREA a Turn off instrument b To remove strip holder tray plate first loosen four screws holding shaking plane c Remove 2 fixing screws from Probe wash basin and detach Waste OUT tube from under basin d Remove connector from shaking plane positioning opto PCB OP Swing PCB 4 fixing screws 2 fixing screws 1 connector ...

Page 22: ... 8 screws from bottle separation carter f Remove 7 screws from aluminium shaking plane g Lift up and detach screws holding the shaking plane movement mechanism motor h NB each time shaking plane support is moved or removed calibrate shaking plane as described Para 4 8 4 Aluminium shaking plane Bottle separation carter ...

Page 23: ...MDS AP 26 00 02 Rev 01 Date 02 04 2009 Page 23 3 2 2 Removing shaking plane a Turn off device b Remove 8 screws from strip holder tray plate c Remove strip holder tray plate d Lift up shaking plane 8 fixing screws strip holder tray plate Shaking plane OP Swing PCB ...

Page 24: ...rators controls and all reagents and also cleans inside of Z1 probe 3 3 1 1 Dilutor Unit The DILUTOR UNIT is composed of following parts clockwise direction 1 Dilutor mechanism Moves probe with step motor 2 2 way Electro valve For the aspiration of washing solution or for probe aspiration dispensation 3 Syringe To aspirate and dispense Dilutor movement mechanism INTERFA2 PCB Syringe SIDILOP PCB Va...

Page 25: ... along a track 6 on 4 ball bearings A shaft is mounted on the carriage 7 which then moves the syringe plunger 1 The optic sensor 8 is mounted on the SIDILOP PCB and signals when the syringe plunger has reached the End line home position 3 3 1 3 Valve mechanism Syringe The valve mechanism connects the Wash bottle to the rest of the hydraulic syringe probe circuit through the peristaltic pump The sy...

Page 26: ...nit The pump unit is made up of 2 peristaltic pumps composed of rotating wheel stand peristaltic pump tubing step motor Note Pump A washes dispensation probe internally and externally and regulates WASH liquid flow during STRIP WASH phase Peristaltic pump tubing Peristaltic pump A Peristaltic pump tubing Peristaltic pump B ...

Page 27: ...e variations of the electrical capacity of the circuit which consists of probe liquid ground The sensor circuit emits a low signal level 0 until the probe touches the liquid A high signal level 1 is emitted to indicate when the probe touches the liquid for duration T in proportion to the variation of the circuit capacity Adjusting probe sensitivity is based on the duration bandwidth of this impuls...

Page 28: ...which senses when syringe has reached end line home position INTEROP2 PCB Support for liquid level sensor and cable distribution INTERFA2 PCB Connects wire cables Liquid Level Sensor Electronic circuit in a small container mounted on probe which signals contact with liquid surface level Syringe Motor A step motor which moves syringe plunger with special worm screw inside the plastic nut Dilutor 2 ...

Page 29: ...quid 5 litre capacity Level Sensor Sensor for the three different bottles and connecting cables and connectors Probe washing pump Peristaltic pump A for washing probe internally and externally driven by step motor Probe wash basin For external probe wash and draining any excess liquid Syringe For aspirating and dispensing liquid through probe Peristaltic Pump A Peristaltic Pump B Syringe Dispensat...

Page 30: ...inge bottom If condition is unsatisfactory substitute plunger following procedure described in para 4 8 5 Syringe Check that syringe glass is in good condition If any damage can be seen then substitute syringe following procedure described in paragraph 4 8 5 Peristaltic pump tubing Check that tubing is in good condition and more specifically check that there are no traces of rubber around pump are...

Page 31: ...tuting 3 way valve mechanism Ref para 3 3 3 4 Substituting Peristaltic Pump Tubing Ref para 3 3 3 5 Substituting Dilutor Motor Ref para 3 3 3 6 Substituting Opto SIDILOP PCB Ref para 3 3 3 7 Substituting INTERFA2 PCB Ref para 3 3 3 8 Substituting probe wash basin Ref para 3 3 3 9 3 3 3 1 Substituting valve syringe dispensation probe tubing a Disconnect AWG15 tubing from probe b The old tubing to b...

Page 32: ...m g Remove valve connector by undoing fixing screw h Remove valve mechanism by undoing 2 fixing nuts i Insert new valve mechanism and re block it with 2 fixing nuts j Replace valve and connector k Replace hydraulic connections l Replace syringe in movement shaft and screw it back into valve mechanism in a clockwise direction m Turn on instrument again and run a prime to rinse hydraulic circuit 3 3...

Page 33: ...their bottles c Set prime for at least 60 seconds to empty hydraulic circuit Select Prime d After this prime rinse detach tubing and then slip tubing off pumps B A e Place new peristaltic tubing into position and re connect it up following the diagram sticker on device f Reconnect both Wash loading and Buffer tubing to their respective bottles g Run another prime by selecting Prime option to rinse...

Page 34: ...tand c Remove connector that joins dilutor motor to INTERFA2 PCB d Loosen two grub screws that block motor axis mechanism e Remove the motor by loosening the 4 fixing screws f Re position new motor and block it in position with 4 motor fixing screws and tighten grub screws that were previously loosened in step d g Restore motor and INTERFA2 PCB connections two grub screws 4 syringe motor fixing sc...

Page 35: ...CB a Remove AWG15 tubing b Remove 4 screws from dilutor panel c Remove connectors from INTERFA2 PCB and two screws attaching it to the plate d Substitute PCB and re attach it with the 2 screws follow numbering to re position connectors correctly e Replace dilutor unit in position 3 3 3 9 Substituting probe wash basin a Unscrew probe wash basin from work area b Lift up basin and disconnect Waste OU...

Page 36: ...he carriage 2 The movement is generated by a motor 3 and a pulley 4 a serrated belt 5 and an idle wheel 6 The XGOLAV opto PCB is mounted on the X axis carriage 1 The encoder 7 controls the position along X axis through encoder slits The Y axis mechanism moves carriage along Y Axis 1 on which the Z1 and Z2 axis are fixed The movement is generated by step motor 2 with a serrated belt 3 and an idle w...

Page 37: ... Y axis XGOLAV X PCB Controls opto with which the encoder controls axis position INTEROP2 Y PCB Opto couplers The Opto couplers together with the selectors control 1 Y probe position 2 Z1 and Z2 probes position 3 ZZ probe position INTERFA1 PCB Connectors for wiring INTERFA2 PCB Connectors for wiring AP22MOT PCB Connectors for wiring X axis motor Step motor Y axis motor Step motor DILAP21 PCB SIR4M...

Page 38: ...uring their movement check that belts are not too tight Ref Para 3 4 3 1 3 4 2 3 Check condition of flat cables a Check that flat cables are not touching any metal parts b Check that cables maintain their original folds 3 4 3 CALIBRATING X Y AXIS SETTINGS To center X Y axis refer to para 4 8 1 The following table lists the relevant paragraphs describing the different possible calibrations Errore L...

Page 39: ...4 4 4 Substituting Flat Cables Ref para 3 4 4 5 Substituting X Y axis movement flat cables Ref para 3 4 4 6 Substituting INTEROP2 PCB Y axis movement Ref para 3 4 4 7 3 4 4 1 Substituting X axis Movement Motor a Remove protective cover from device by loosening the screws on top b Remove motor connector from INTERFA2 PCB c Loosen 2 dowels on wheel which sustains the belt d Remove motor by loosening...

Page 40: ...e new motor in position and fix in place with 4 screws e Check belt tension f Reconnect motor to AP22MOT PCB with connector 3 4 4 3 Substituting Opto XGOLAV X axis movement PCB a Remove top cover after undoing screws on top b Remove connector attached to XGOLAV PCB c To substitute it unscrew PCB from plate d Place new PCB in position and screw it to the plate with two fixing screws e Re insert con...

Page 41: ... cable and replace all previous cable holder bands e Carefully re insert connectors in correct positions 3 4 4 6 Substituting X Y axis movement flat cables a Remove device top cover after undoing top screws b Remove connectors between Y axis motor and AP22MOT PCB c Remove all plastic cable holder bands d Remove cable blocking plates PIASTRINE CHE BLOCCANO I CAVI DI MOVIMENTO e Dismount INTERFA1 PC...

Page 42: ...OP2 PCB Y axis movement a Undo fixing screws to remove device top cover b Detach connectors from INTEROP2 PCB and remove 4 fixing screws c Reposition new PCB and secure it with 4 fixing screws d Reinsert connectors e Check Y axis centering f Check Z maximum value g Reposition cover and screw back screws 2 fixing screws 2 fixing screws ...

Page 43: ...on mounted on its shaft 2 that transmits the movement to the carriage 3 The optical sensor by means of the carriage encoder 4 detects the probe End line home position FUNCTION OF Z2 AXIS aspiration Z2 Axis moves the aspiration probe up and down over work area The movement is driven by a step motor 1 with a pinion mounted on its shaft 2 that transmits the movement to the carriage 3 The optical sens...

Page 44: ...ollowed by a brief description and the following table lists the various PCBs and their components DILAP21 PCB Contains the microprocessor memory and Z axis motor drivers INTEROP2 PCB Contains optical sensor to detect probe end line run home position Z1 Z2 axis motor Step motor which moves Z1 Z2 axis probes DILAP21 PCB INTEROP2 PCB Liquid sensors Memory Microprocessor M Z2 Axis motor Z Axis motor ...

Page 45: ...obe 1 Select Go down option b Check that movements are smooth and linear c Repeat movement control also on probe 2 d If movements are not smooth or and linear check pinion or carriage and replace parts if necessary e If any parts are substituted verify movements as in point b 3 5 3 Z AXIS CALIBRATION To calibrate Z axis refer to para 4 8 The following table lists the relevant paragraphs describing...

Page 46: ... 1 Substituting Z1 Axis probe a Remove instrument cover b Remove protective carter by unscrewing two fixing screws c Detach AWG15 tubing from Probe d Remove liquid sensor cable connector from INTEROP2 PCB e Remove screw that fixes probe to stand f Replace probe with new probe and tighten fixing screw g Reconnect probe and liquid sensor connector NON CE SU VERSIONE ITaliana to INTEROP2 PCB and repo...

Page 47: ...tween PCB and motor re position cable holder bands f Check that Z axis movements are smooth g Check Z1 axis steps h Check X Y axis centering 3 5 4 3 Substituting Z2 Axis probe a Remove silicon tubing connected to probe b Unscrew probe fixing screw c Replace probe and tighten fixing screw d Check centering of dispensation probe e Check distance between probes in home position HH command with DIL PC...

Page 48: ...tive carter after undoing two fixing screws b Remove motor connector from INTEROP2 PCB and remove cable holder bands c Remove fixing screws and change motor d Reconnect motor to PCB and replace cable holder bands e Check that Z2 axis movement is smooth f Check Z1 movements and steps g Check X Y centering as described in para 3 4 3 1 2 fixing screws Motor fixing screws ...

Page 49: ...s shakes the slides with the strips to be processed The slides are placed on the hinged shaking plane 1 and the movement is driven by a worm screw 2 on a ball bearing 3 which moves along a wedge 4 mounted under the shaking plane The ball bearing moves back and forth on the wedge generating the up and down movement of the plane The minimum and maximum shaking excursion is controlled by 3 opto posit...

Page 50: ...that move the Shaking plane This is followed by a diagram of the mechanism APE IF DIL AP21 PCB Z1 and Z2 axis motor drivers OP SWING PCB Shaking plane optical sensors that control movement Shaking plane Motor Step Motor that moves worm screw and ball bearing for shaking plane movement DILAP21 PCB INTERFA7 PCB OP SWING PCB M Oscillating plane motor Shaking plane motor driver Down Home Flat position...

Page 51: ...BSTITUTING SHAKING PLANE COMPONENTS Substituting Shaking Plane Motor Para 3 6 3 1 Substituting opto PCBs Para 3 6 3 2 3 6 3 1 Substituting Shaking Plane Motor a Remove aluminium work area as described in Para 3 1 b Estrarre il piano di lavoro in alluminio c Loosen two nuts blocking motor axis d Undo 4 fixing screws to remove motor e Position new motor replace and tighten 4 fixing screws and nuts f...

Page 52: ...bstituting opto PCBs a Remove work area as described in Para 3 2 1 b Detach connector from Opto PCB to be substituted c Remove fixing screws to undo PCB d Reposition new PCB check that the Opto is not touching the encoder and replace the connector e Reposition work area f Calibrate shaking plane Connector Fixing screws ...

Page 53: ...p These images are then automatically elaborated by the software and each single blot is analysed The camera is mounted on a stand 1 next to the probe unit The Optical Unit is made up of the camera 2 and lens 3 The lens is screwed on the camera with a 5mm mount 4 There are two rings with screw threads one can be turned to focus 5 and the other 6 regulates the aperture For camera calibration see Pa...

Page 54: ...cloth lightly dampened with alcohol 3 7 3 CALIBRATE CAMERA To calibrate camera refer to para 4 8 9 The table below lists the calibration procedures described in the relevant paragraphs 3 7 4 SUBSTITUTING CAMERA AND OR PARTS Substituting camera Para 3 7 4 1 Substituting camera lens Para 3 7 4 2 Substituting camera USB cable Para 3 7 4 3 Substituting LED light bar Para 3 7 4 4 3 7 4 1 Substituting c...

Page 55: ...d in Para 4 8 9 3 7 4 3 Substituting camera USB cable a Turn off device b Undo fixing screws above cover and then remove cover c Open left flap d Detach camera USB cable e Carefully pull out cable from X and Y axis plastic box chain f Detach USB connector from the USB hub on the left flap g Pull out USB cable h Insert new cable by repeating above procedures in reverse from point g to point a i Cal...

Page 56: ...d for detecting the position of the first well or first filter Encoder zero position 1 2 1 well position 1 Zero LED 2 Signal LED 3 8 2 REGULAR MAINTENANCE 3 8 2 1 Cleaning opto Clean opto sensors around every 6 months using a soft brush to remove any traces of dirt or dust 3 8 2 2 Checking procedure The various opto PCBs function in different units The following table lists these different units t...

Page 57: ... signal LED 0 zero LED 1 X signal LED 0 zero LED 1 X signal LED 0 zero LED 1 X signal LED 0 zero LED 1 X signal LED 0 zero LED 1 X signal LED 0 zero LED 1 X signal LED 0 zero LED 1 X signal LED 0 zero LED 1 X signal LED 0 zero LED 1 X signal LED 1 zero LED 1 X signal LED 1 zero LED 1 X signal LED 1 zero LED 1 X signal LED 1 zero LED 1 X signal LED 1 zero LED 1 X signal LED 1 zero LED 1 X signal LE...

Page 58: ... signal LED 0 zero LED 1 Y signal LED 0 zero LED 1 Y signal LED 0 zero LED 1 Y signal LED 0 zero LED 1 Y signal LED 0 zero LED 1 Y signal LED 0 zero LED 1 Y signal LED 1 zero LED 1 Y signal LED 1 zero LED 1 Y signal LED 1 zero LED 1 Y signal LED 1 zero LED 1 Y signal LED 1 zero LED 1 Y signal LED 1 zero LED 1 Y signal LED 1 zero LED 1 Y signal LED 1 zero LED 0 Y signal LED 1 zero LED 0 Y signal LE...

Page 59: ...rough a USB connection COMPOSITION DILAP21 PCB USB Personal Computer 3 9 2 CHECK FUNCTIONALITY a Start TCom program and set up communication port type VE to display Dilutor PCB firmware version b If access to Control Panel is not possible then follow the connection diagram as follows PCBs APE IF DIL AP21 connector J15 c If connection sequence is correct then substitute the PCB which is not communi...

Page 60: ...0 3 9 3 REPLACING DILAP21 and SIR4MOT PCBs a To remove right back panel undo two fixing screws b Locate DILAP21 or SIR4MOT2 PCBs and remove all connectors c Undo 4 fixing screws to remove PCB d Replace PCB and reposition connectors e Close right back panel and replace fixing screws DIL AP21 PCB SIR4MOT2 PCB ...

Page 61: ...DLn1 Moves Z1 until it touches liquid with a maximum number of motor steps defined as n1 Creates a separation bubble according to the last work phase This bubble is created if number of steps along the Z axis is more than 0 DP Displays all stored values excluding work area values DXn Moves Z axis defined by n where n 1 along Z1 axis until the liquid level is reached for a maximum number of motor s...

Page 62: ...ive forwards direction for X Y and Z axis KVhn n Sets calibration factor to correct conversion of microlitres to motor steps for syringe h h 1 KX Moves X axis one motor step The direction is determined by K and K commands KY Move Y axis by one motor step The direction is determined by K and K commands LAL Activates display warning about wash bottle status alarm 0 full 1 empty LAB1 Buffer1 bottle a...

Page 63: ...and brake ramp where a start frequency b stop frequency and c number of ramp elements default a 400 b 4000 c 250 RZa b c Defines acceleration ramp and brake where a start frequency b stop frequency and c number of ramp elements valid for Z1 and Z2 axis default a 200 b 4000 c 200 SA0 Disables bottle alarm SA1 Activates display warning whenever any bottle changes status SB1 Puts logic level 0 the bu...

Page 64: ...valve 1 VOh Closed h valve h 1 syr valve 1 VS Disactivates communication between wash buffer bottle electro valve and wash bottle VUL Displays SUL value command after WB1 Waits for signal 1 from busy 1 of reader washer PCB Wc Enables creation of 10 macro commands c 0 9 with a maximum 250 commands WA Activates the bottle waste valve in aspiration WS Disables the waste valve to aspire from aspiratio...

Page 65: ...rror handling set by SEE command K Shows last typed command P Stops executing commands Q Restarts executing commands R Resets PCB S Displays logic status signal of probe position location T Activates time display one time only through commands WT and TW X Starts executing commands in memory buffer see the WX command Y1 Displays Check1 signal status YL Turns off error LED YR Reset Busy1 YS Set busy...

Page 66: ...0 02 Rev 01 Date 02 04 2009 Page 66 3 11 HYDRAULIC CIRCUIT DIAGRAM Peristaltic Pump A Peristaltic Pump B Syringe Dispensation probe Aspiration probe Needle washing basin WASTE bottle WASH BUFFER bottle WASH bottle WASH WASH BUFFER WASTE 3 way Electrovalve 3 way Electrovalve ...

Page 67: ...rts refer to paras 4 7 1 and 4 8 8 The next table shows the sections dealing with routine maintenance checks Prime Para 4 8 8 Tank level Sensors Para 4 7 1 3 11 2 SUBSTITUTING HYDRAULIC CIRCUIT COMPONENTS 3 11 2 1 Electro valve substitution a Remove front work area b Locate electro valve to be changed c Disconnect tubing and remove valve d Position new valve and reconnect everything ...

Page 68: ...ables Every 6 months Para 3 4 2 3 3 12 3 Z2 AXIS Operation How often Reference Z1 Z2 axis movement mechanism Every 6 months Par 3 5 1 1 3 12 4 SHAKING PLANE Operation How often Reference Calibrate shaking plane Every 6 months Para 4 8 4 3 12 5 CAMERA Operation How often Reference Checking USB cable connection Every 6 months Para 3 7 2 1 Cleaning lens Every 6 months Para 3 7 2 2 3 12 6 OPTICAL SENS...

Page 69: ...AP Blot SERVICE MANUAL SECTION 4 CALIBRATION PROCEDURES XCalib Doc N MDS AP 26 00 02 Rev 01 Date 02 04 2009 Pag 69 SECTION 4 CALIBRATION PROCEDURES XCALIB ...

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Page 71: ...ge English or Italiano Please Note this selection is only valid for installation Click Next Insert User Name and Company Name Click Next Select where installation file should be saved The Default path is C Programmi APEXSuite folder Click Next Select programme to be installed for this procedure it is XCALIB Click Next and then Install When software has been installed then install USB driver as des...

Page 72: ...ngs Tank level sensors Print Print preview Movement test Aspiration test Dispensation test Calibrate height Calibrate probe position Language About Calibrazione LT Exit Close Turn on lights Home Reset Turn off motors Show Calibration Settings Calibrate Camera Prime Wash Basin Test Calibrate SUL Calibrate probes Calibrate shaking Serial Number Communication Port Hardware Settings Software component...

Page 73: ... levels LT Calibrate shaking plane Calibrate shaking plane Calibrate syringe Removing syringe check and calibrate syringe tip positions check aspiration and dispensation Calibrate SUL Tests and calibrates SUL settings Test wash basin Tests peristaltic pump and electro valve workings Prime Primes dilutor unit hydraulic circuit Calibrate camera Calibrate camera Calibration settings Displays calibrat...

Page 74: ...s off all motors Calibrate probe height Calibrates Z maximum height Turning lights on Turns on off internal lights Hardware configuration Accesses hardware configuration Print Preview Displays calibration settings Print Prints calibration settings Dilutor parameter report Displays dilutor PCB calibration settings Close function Closes active function and opens main display Reset Stops current acti...

Page 75: ...re configuration or directly click on icon on main instrument bar The following display will appear This display can be used to set up the following which path the XML folder will be associated with this configuration which can be chosen from a list by clicking Browse button which instrument configuration can be calibrated from the pull down menu which type of configuration will be associated with...

Page 76: ... Here choose which language is displayed Italian English 4 5 3 Communication port From Settings menu select Communication port The following display will appear This display shows the possible active communication ports open by the PC from a pull down menu Confirm communication port by clicking on SET and the programme displays a confirmation message for the User ...

Page 77: ...4 Serial number From Settings menu select Serial Number The following display will appear Click on Current value to display actual number setting To change serial number type in new number and click on Store and when this new number has been successfully stored the programme displays a confirmation message for the User ...

Page 78: ...d Work Area lay out The Work Area is Disactivated when whole layout is displayed Activate when a specific part of Work Area is selected to return to overall lay out of Work Area as shown Probe washing basin Strip holder incubation tray Additional reagent rack Reagent and control rack 48 position sample rack Disactivated Work Area command Active Work Area key ...

Page 79: ...nds and then displayed To check that sensors are correctly functioning 1 Take off cap 2 Turn cap upside down to lower the level sensors white cylinder 3 Check condition of tanks Not full not empty which is updated each time the sensor moves up or down If no sensor update occurs then check the following Check that level sensor floater is not blocked or obstructed Check that level sensor cables are ...

Page 80: ... Movement Test to check that the different moving parts are functioning correctly This opens the following display Click on Start to run test The test automatically checks movement in the X Y Z axis the shaking plane movement primes hydraulic circuit and checks communication with the camera If all the controls are positive at the end of the test the following display appears ...

Page 81: ...nnections are correct belt tension is correct ref para 3 4 2 2 relevant motor is functioning correctly relevant PCB is functioning correctly If Dilutor Error Z Axis movement 1 2 probe failed appears check that relevant connections are correct relevant motor is functioning correctly relevant PCB is functioning correctly If Dilutor Error Blot Shaker failed appears check that relevant connections are...

Page 82: ...n menu select Print preview Print preview can only be selected to display the section of the Calibration function which is currently active This function is only active when a calibration is taking place 4 7 6 Print From Main Functions pull down menu select Print The Print function is enabled only when Calibrate function is active and prints out current functions ...

Page 83: ... are functioning correctly for reading phase If light does not come on check and possibly substitute the following in order 1 connections are correct 2 2 Colight PCB 3 3 LED light bar see ref para 3 7 4 4 4 7 8 Close Function From Main functions pull down menu select Close function to close active functions and return to initial display 4 7 9 Exit From Main Functions pull down menu select Exit to ...

Page 84: ... Dilutor menu select Calibrate probe positions The following display will appear Any part of the Work Area can be selected with a mouse click on it The probes will automatically move into the first position over the selected area Different commands of panel are activated disactivated depending on which section of the Work Area is selected Work Area Command Panel ...

Page 85: ...ce is incorrect manually re position probe 2 When procedure is completed return to Home position Centering probes 1 and 2 Check and calibrate probe positions over work area Checks It is advisable to calibrate using one of the smaller diameter phials for the sample and reagent areas Here is the standard calibration procedure for the Work Area 1 Select Sample Rack from Work Area display 2 The first ...

Page 86: ... device as shown in fig 1 1 Remove device top cover 2 Loosen fixing screws on APX XGOLAV X PCB 3 Move PCB manually to bring the probe directly over the center of the reference point and then tighten the screws on the PCB 4 Select Go up to lift probe up and then click on icon to turn off motors 5 Move arm that controls optic sensors so that it now moves freely over the encoder 6 Return to Home posi...

Page 87: ...ends of the encoder 3 Move encoder manually until the probe is in the centre of the reference point and then tighten the screws 4 Select Go up to bring probe up and turn off motors 5 Move the probe unit along the Y axis to check that Optic sensors move freely along the encoder 6 Return to Home position 7 Select Go down and check that probe is correctly centered 8 Repeat procedure from point 2 unti...

Page 88: ...n both positions continue with calibration procedure as described Calibration 1 Select first strip holder incubation tray Tray 1 2 Select probe 2 from command panel and click on Go down 3 Loosen the four fixing screws on the base of the shaking plane support Fig 2 Fig 2 4 Move the base of the stand to regulate position of probe and center it in aspiration well 5 Return to Work Area and select last...

Page 89: ...leaning solution area and C1 control area 3 By selecting sample rack in Work Area position 1 and the last stored maximum height value are also automatically selected 4 Select Go down command The probe will go down the number of steps shown in the Height box 5 Now check if probe is If probe is touching bottom of well type in 100 less steps from the current value displayed in Height box Repeat proce...

Page 90: ...alue of 800 steps in Height box 10 Select Go down and probe will go down the number of steps shown in Height box The shaking plane will tilt as set up in Plate Slope pull down menu 11 Check probe position If probe is touching bottom of well manually type in 100 steps less than initial value in the Height box Repeat procedure from point 4 If probe is NOT touching bottom of well select the Increase ...

Page 91: ...ted 2 Prepare test solution distilled water and dilutor liquid in 1 100 ratio 3 Insert test tube with 200µl liquid sensor solution anywhere in sample rack 4 Remove probe 1 tube 5 From Sample number box type in or pull down value of position where test tube with liquid is inserted 6 Select Measure LT command 7 The probe will automatically go down 10 steps inside the test tube to measure and evaluat...

Page 92: ...re LT Value advised by software is equal to half of the minimum sensitivity measurements For standard Calibrations this value should be set up in LT box Select Close function icon to return to initial display and copy the LT parameter to set up in LT box Select Store Check LT value setting To check LT setting on different types of phials select a section of Work Area insert phial used with at leas...

Page 93: ...default value is 6 and select Start shaking To interrupt movement select Reset and the shaking plane goes back to home position flat 2 Check that shaking movement is smooth and not obstructed Click on Move up and Move down commands to check that up and down movements are correct The shaking plane will automatically move within the range as set up minimum 1 and maximum 9 3 If movement is not smooth...

Page 94: ... all strip holder plates from shaking plane and use a spirit level to check that shaking plane is level both for X and Y axis Regulate Swing PCB opto under shaking plane if not level ref para 3 3 6 1 Horizontal Position Calibration Remove Plate Holder Fig X Life off shaking plane and loosen fixing screws of the OP Swing PCB Fig X Move the PCB to mechanically regulate the shaking plane home positio...

Page 95: ...v 01 Date 02 04 2009 Pag 95 4 8 5 Calibrate syringe From Dilutor pull down menu select Calibrate syringe The following display will appear Calibration This procedure aims to regulate the distance between the syringe Tip and syringe mount is circa 1mm fig 1 Fig 1 Syringe Tip syringe mount ...

Page 96: ...lay to empty whole hydraulic circuit 3 Select Syringe Removal key The plunger lowers and the syringe can be removed by twisting it anticlockwise 4 Insert new syringe Twist it clockwise back into syringe valve unit select Reset syringe 5 Check Tip Position by referring to Calibration procedure described earlier 6 Reconnect WASH and WASH Buffer 1 tubes Substitute plunger 1 Disconnect Wash and Wash B...

Page 97: ...l move down to just above the liquid and will aspirate the set volume of liquid 9 On the work area display select the empty test tube position on sample rack 10 Select Dispensation key the probe will move to selected position and go down to Z maximum height and dispense the set amount of liquid 11 Check with a teat pipette that the amount of dispensed liquid corresponds to amount set up previously...

Page 98: ...is not full and that WASH tank is not empty 3 Check Prime box to activate Prime function 4 Set up the duration of hydraulic circuit Prime recommended 20 seconds in Prime duration box 5 Input 100 as first value for SUL set up in SUL box 6 Select Test Liquid volume as selected should be dispensed in test tube 7 Check with a teat pipette that 1ml of liquid has been dispensed through valve opening SUL...

Page 99: ...ck that Waste basin is not full and that Wash basin is not empty 2 Select Waste pump on and check that peristaltic pump B turns in clockwise direction 3 Select Waste pump off 4 Select Wash pump on and check that peristaltic wheels A and B are turning and that no liquid comes out of probe 1 Select Wash pump off If part of this procedure does not function correctly check that Check that peristaltic ...

Page 100: ...be valve 4 Check that probe 1 dispenses liquid continuously and that wash basin correctly aspirates liquid through the pump 5 Select Close probe valve 6 Check that probe 1 and electro valve are not dripping 7 Select Wash pump off If this procedure is not successful Check that electro valve is mounted correctly Substitute electro valve Check Wash Basin functioning 1 Ensure that Electrovave is close...

Page 101: ...elected then remove Wash Bottle cap 4 If Wash is selected then remove top to Wash Buffer 1 bottle 5 Input amount of seconds for duration of prime in box 6 Select Prime and check that liquid is aspired from previously selected bottle If any part of this procedure does not function correctly check that Upper and lower tubes are correctly connected to the wash tank cap Electro valve A can correctly s...

Page 102: ...st correct gain calibration 1 Check that internal light is on instrument cover is closed and top cover is inserted 2 Select Auto Gain ON The camera will then move to frame the Calibration Strip as shown in Fig 1 and will automatically set a white balance 3 After this balance check Gain Value 4 If Gain Value is between 40 and 60 the diaphragm is perfectly calibrated If not then Calibrate Gain 5 Sel...

Page 103: ...Gain OFF to interrupt Calibration process 10 A correction value shown between brackets will be subtracted from the obtained value to be then stored and displayed O C Near Far Fig 2 Correct Focus Test 1 Select Auto Gain ON Wait for white balance 2 Select Auto Gain OFF 3 Check that camera focus is correct with reference to numbers on Calibrating strip Fig 1 4 For a perfect calibration ensure that nu...

Page 104: ...ormance The Check PC performance command runs an automatic test to check that the PC has sufficient attributes to be used with these devices At the end of the test the result is displayed Pixel Clock The value displayed in the Pixel Clock field is relative to the characteristics of the PC being used if they are sufficient then the pixel clock display is over 24 4 8 10 Calibrate parameters From Dil...

Page 105: ... Home From Dilutor pull down menu select Home Probes are returned to Home position over probe washing basin 4 8 12 Turning motors off From Dilutor pull down menu select Turning motors off All motors are turned off and can now be moved manually 4 8 13 Reset From Dilutor pull down menu select Reset All the PCBs are now Reset ...

Page 106: ... following display will appear This display shows information about the firmware installed If any function failure occurs then an Error message is displayed e g if communication with the instrument is not successful In this case check that all DILAP21 PCB connectors are correctly connected specifically J15 connector If all these connections are correct then substitute PCB see ref para 3 8 3 ...

Page 107: ... component information From menu select Software component information The following display will appear This display shows the different software versions in use 4 9 3 About From menu select About The following display will appear This display shows the name of the programme the programme version and manufacturer contact details ...

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Page 109: ...AP Blot SERVICE MANUAL SECTION 5 TROUBLE SHOOTING Doc N MDS AP 26 00 02 Rev 01 Date 02 04 2009 Pag 109 SECTION 5 TROUBLE SHOOTING ...

Page 110: ......

Page 111: ... in aspirated sample Probe tubing or valve are dirty or worn out Replace probe tubing or valve f False alarm of lack of liquid Liquid sensor not sensitive enough Regulate LT parameter or replace probe liquid sensor damaged g Tip stops before contacting liquid Liquid sensor too sensitive Regulate LT parameter or replace probe liquid sensor damaged h Syringe does not aspirate liquid Air in circuit C...

Page 112: ...el on Z axis motor b Dispensation Probe misses steps Wheel track is dirty Clean surface of wheel track c Probe does not move Motor or circuit control worn out Check motor wiring and Dilutor card and replace parts if necessary d Z2 motor misses steps Motor gear too far from its rack Regulate motor screws to bring motor nearer to rack SHAKING PLANE PROBLEM PROBABLE CAUSE SOLUTION a Cables are not co...

Page 113: ...AP Blot SERVICE MANUAL SECTION 6 SCHEMATIC DIAGRAMS Doc N MDS AP 26 00 02 Rev 01 Date 02 04 2009 Pag 113 SECTION 6 SCHEMATIC DIAGRAMS ...

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