BERTHOLD TECHNOLOGIES NightOWL II LB 983, Operating Manual

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LB 983 NightOWL II Operating Manual Appendix
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10.1.7 Test Light Source
The Test Light Source is used to calibrate and test the System..
It contains a low light source which will glow for about 15 min. without external supply. To
charge the unit, use the included Mains adapter. Charging is indicated by a red LED on the test
plate. As soon as the plate is fully charged, the green LED will light. It takes about 1-2 minutes
to charge the plate.
Now the plate is ready to be used in the NightOWL. The spot with the white reflector (microplate
position E9) emits light with an energy of approx 20nW. This spot can be used to check the
NightOWL calibration. The measuring time should not exceed 1 second since the camera could
be saturated otherwise.
Position E6 gives a lower signal intensity. This position can be used to check the detection limit
with additional neutral density filters on top of the light source.
The sample size should be 130mm with a sample height of 14mm. A special center plate is op-
tionally available to place the E9 light spot at the center of the image.
The required light position is controlled though the sliding switch at the side of the plate (position
E9 -> overl.; E6 -> 96). When the plate is not in use put the slider switch to position
„OFF“.