Beckman Coulter UniCel DxC 660i, Training Manual

Page: 15 / 103

DxC Integrated Workstation In-Lab Training Manual Page 15 of 101
Rev. A (June 2014) Beckman Coulter Professional Development

If the sample needs to be tested at the DxI analyzer:

A sample container is pierced by a piercer probe (5) if a cap is present

Sample is aspirated by an aliquot probe (5) and delivered to an empty sample
vessel (SV) on the UCTA SV shuttle (6)

The SV shuttle moves to the UCTA pick and place (7)

The UCTA pick and place transfers the SV from the SV shuttle to the UCTA SV
nest (8)

The UCTA pick and place transfers the SV from the SV nest to UCTA transfer
shuttle (9)

The UCTA transfer shuttle moves to the DxI pick and place (10)

The DxI pick and place transfers the SV from the UCTA transfer shuttle to the
DxI sample storage (11)

Empty SV(s) from the DxI sample storage are transferred to the SV shuttle for
the next sample aliquot(s)

DxI analysis begins

The SV is used as the sample for immunoassay testing

A reagent pipettor delivers reagent and sample to a reaction vessel (RV)

The RV is incubated then washed to remove unbound material; substrate is
added to the RV for the chemiluminescent reaction

The luminometer measures the amount of light generated from the RV; the
measure light is converted to assay concentration

The UCTA shuttle moves the sample rack to the DxC load area.

If the sample needs to be tested at the DxC analyzer:

The sample rack is delivered to the DxC sample carousel.

DxC analysis begins

If the sample requires MC testing:

Sample is delivered to the MC reaction cups modules for reaction cup
analysis and to the EIC for electrolyte analysis

If the sample required CC testing:

The CC reagent probes deliver reagent to a cuvette; the reagent is
warmed and photometer takes reagent blank readings

Sample is delivered to the cuvette

The photometers takes reaction readings; the readings are converted to
analyte concentration

The cuvette wash station washes, rinses, and dries the cuvette for resuse

When the sample is no longer needed for testing, the DxC shuttle moves the
sample rack to the DxC off-load area.