Chapter 2
Design the Relative Standard Curve Experiment
Review the Reaction Setup
Applied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Relative Standard Curve
and Comparative C
T
Experiments
40
Notes
To complete the Sample Dilution Calculations tab:
1.
In the Reaction Setup screen, select the
Sample Dilution Calculations
tab.
2.
Click the
Diluted Sample Concentration (10
✕
for Reaction Mix)
field, then
enter
50
.
3.
In the unit drop-down list, select
ng/
µ
L
(default).
4.
Review the calculated volumes for the sample dilutions:
7b
7a
7c
Sample Name
Stock Concentration
(ng/µL)
Sample Volume
(µL)
Diluent Volume
(µL)
Total Volume of Diluted Sample
(µL)
Liver
100.0
17.00
17.00
34.00
Kidney
100.0
17.00
17.00
34.00
1
2
3
4
