Glossary
223
Applied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Relative Standard Curve
and Comparative C
T
Experiments
regression
coefficients
Values calculated from the regression line in standard curves, including the R
2
value, slope,
and y-intercept. You can use the regression coefficients to evaluate the quality of results
from the standards. See also standard curve.
regression line
In standard curve and relative standard curve experiments, the best-fit line from the
standard curve. Regression line formula:
C
T
= m [log (Qty)] + b
where m is the slope, b is the y-intercept, and Qty is the standard quantity.
See also regression coefficients.
reject well
An action that the software performs during analysis to remove one or more wells from
further analysis if a specific flag is applied to the well. Rejected wells contain results
calculated up to the point of rejection.
relative standard
curve method
Method for determining relative target quantity in samples. With the relative standard curve
method, the 7500 software measures amplification of the target and of the endogenous
control in samples, in a reference sample, and in a standard dilution series. Measurements
are normalized using the endogenous control. Data from the standard dilution series are
used to generate the standard curve. Using the standard curve, the software interpolates
target quantity in the samples and in the reference sample. The software determines the
relative quantity of target in each sample by comparing target quantity in each sample to
target quantity in the reference sample.
replicate group
A set of identical reactions in an experiment.
replicates
See technical replicates or biological replicates.
reporter
Fluorescent dye used to detect amplification. If you are using TaqMan
®
reagents, the
reporter dye is attached to the 5
′
end. If you are using SYBR
®
Green reagents, the reporter
dye is SYBR
®
Green dye.
reverse primer
An oligonucleotide that flanks the 3
′
end of the amplicon. The reverse primer and the
forward primer are used together in PCR reactions to amplify the target.
reverse
transcriptase
An enzyme that converts RNA to cDNA. Reverse transcriptase is added to the PCR reaction
to perform 1-step RT-PCR.
Rn
See normalized reporter (Rn).
ROX
™
dye
A dye supplied by Applied Biosystems and precalibrated on the 7500/7500 Fast systems.
ROX dye is used as the passive reference.
rs number
See refSNP ID.
run method
Definition of the reaction volume and the thermal profile for the 7500/7500 Fast instrument
run.
sample
The template that you are testing.
