Chapter 9
Analyze the Comparative C
T
Experiment
Analyze the Study
197
Applied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Relative Standard Curve
and Comparative C
T
Experiments
Notes
The Well Results Data group displays data for each well in the reaction plates that are
added to the study, including:
• The sample name, target name, task, and dyes
• The calculated threshold cycle (C
T
), normalized fluorescence (Rn), and quantity values
• Flags
About the
Example Study
In the comparative C
T
example study, you review:
• Each target in the Gene Expression Plot screen for the expression level (or fold
change) of the target sample relative to the reference sample.
• The Replicate Results Data group to evaluate the precision of the replicate groups.
View the Gene
Expression Plot
1.
In the navigation pane, select
Analysis
Gene Expression
.
2.
In the Gene Expression Plot screen:
a.
In the Plot Type drop-down list, select
RQ vs Sample
.
b.
In the Graph Type drop-down list, select
Log10
.
c.
In the Orientation drop-down list, select
Vertical Bars
.
3.
Click
(Show a legend for the plot).
Note:
This is a toggle button. When the legend is displayed, the button changes to
Hide the plot legend.
In the example experiment, the expression level of multiple targets in samples are
displayed relative to the expression level of the same targets in the reference sample
(universal). Because the reference sample is compared to itself, the relative expression
level is 1. When the result is displayed in the Log10 graph type, the expression level of
the reference sample appears as 0 in the graph (log10 of 1 = 0).
