Chapter 2
Design the Relative Standard Curve Experiment
Define the Experiment Properties
Applied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Relative Standard Curve
and Comparative C
T
Experiments
22
Notes
Define the Experiment Properties
In the Experiment Properties screen, enter identifying information for the experiment,
select the instrument type, then select the type of experiment to design.
About the
Example
Experiment
In the relative standard curve example experiment:
• The experiment is identified as an example.
• The instrument that is selected to run the experiment is the 7500 instrument.
• A MicroAmp
®
Optical 96-Well Reaction Plate is used.
• The experiment type is quantitation.
Complete the
Experiment
Properties Screen
1.
Click the
Experiment Name
field, then enter
Relative Standard Curve Example
.
Note:
The experiment header is updated with the experiment name that you enter.
2.
Leave the Barcode field empty.
3.
Click the
User Name
field, then enter
Example User
.
4.
Click the
Comments
field, then enter
Example Experiment for the Relative
Standard Curve Getting Started Guide
.
5.
Select
7500 (96 Wells)
.
6.
Select
Quantitation
for the experiment type.
7.
Click
Next
.
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