PANNORAMIC® SCAN RX 1.0
SCANNING MODES AND SETTINGS
MAY 13, 2021
97
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES .
Set the correct focus value of the desired preview position by clicking
SetI1
,
SetI2
, or
SetI3.
If you want to go back to any of the previously set focus points, click the appropriate
button. To delete the stored values, click
Clear Values
.
The software calculates a coarse focus level from the three values determined this way as a
basis for the fine focusing routine. You can only define the start parameter here. This will,
however, speed up the focusing process.
If you deselect the
Use fine focus
checkbox, the software immediately starts scanning
based on the interpolated values, without pre-fine-focusing.
To define focus limits, select
Limits of the range
. Set the upper and lower limits by selecting
the desired value in the selection boxes. By using
± Range
, the
Lower limit
value is set to -32
of the current focus value and the
Upper limit
value is set to +32 of the current focus value.
This is useful if
Auto Focus
does not find the focus on the specimen, but on the coverslip.
Also, this makes the focusing sequence faster.
For manual focusing define the manual focus step size by adjusting the
Step size
slider and
use the two arrow buttons to focus manually. This is useful when you are interested in an
area that remained blurry with
Auto Focus
(for example, the specimen is thicker in that
area). This does not influence the way slides are digitized in Auto focus mode.
Figure 82. Focus indicator panel
5.2.2.3
Exposure Indicator
Exposure time for the live image can be calculated by using the
Calculated Exposure time
function
. It is available to switch between microsecond, millisecond, and second units
for the reading of exposure time.
Figure 83. Window of Exposure Indicator in Fluorescence scan mode
Select the types of filters you want to use during scanning. You can select the filter types
and the number of channels to be used. The values in the
EXC
column define the excitation
of the Lumencor illumination, in
TW
define exposure time weight, and in the
DG
the
amount of digital gain is displayed. It is also available for you to enable Extended Focus (
EF
)
and/or Z-stack (
ZS
) functions in this field.