PANNORAMIC® SCAN RX 1.0
SCANNING MODES AND SETTINGS
MAY 13, 2021
77
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES .
5.2
Fluorescence Microscope Mode
Fluorescence Microscope View consist of three different chapters:
•
Profile Settings panel
: Profile settings containing parameters used for scanning
•
Microscope Control Panel
: Live microscope movement functions
•
Main functions
: Located at below the window header, these functions are identical in
both modes.
5.2.1
Profile Settings Panel
Filter Settings
Compact table view of microscope filters used for Fluorescence scanning.
Legend of the table:
•
A
: Actual filter in use
•
Use
: Filters used for viewing Live image and for scanning
•
Auto Exp
: When activated, the exposure time of the selected filter is calculated
automatically during scanning.
•
Exp. Time
: If
Auto Exp
is inactive, exposure time of each filter can be defined
manually. Values such as
Calc Exposure time
in Microscope Control panel and
those that are specified manually are loaded in these fields. Value units can be the
following: microsecond, millisecond, second.
•
Exc %
: The given value specifies the excitation intensity in percentage of the
illumination unit.
•
DG
(Digital Gain): This is camera-dependent, with Point Grey Grasshopper 3 the
index value ranges from 0 to 4, and with pco.edge 4.2 bi from 0 to 6. Give
Index
value by entering it in the box or by selecting the value with the arrow buttons. If
you raise the
Index
value, the exposure time is halved by every step, and the
image becomes noisier. To move between the filters, click
Previous Filter
or
Next
Filter
.
•
TW %
: You can set the
Exposure time weight (%)
here (displayed under
TW
in the
Filter Selection
frame).
•
F
: Focus channel under the scanning
•
S
: The selected channel will be the stitching channel under the scanning.
•
E
/ Z
: Multilayer option: Select
Extended focus
or
Z-stack
for scanning the required
channels in accordance with the scanning mode set in the profile.