PANNORAMIC® SCAN RX 1.0
SCANNING MODES AND SETTINGS
MAY 13, 2021
79
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES .
Figure 62. Focus settings
•
Auto focus:
Focus value is set automatically to match sample characteristics.
•
Limit the range
: You can define focus limits by selecting
Limit the range
for focusing. You
can set the upper and lower limits by selecting the desired value in the selection boxes.
This is useful if focusing was not targeted on the specimen, but on a spot on the coverslip.
•
Focus frequency:
This value determines how many fields of views will pass before the
sample is refocused. This value should be selected depending on the unevenness and size
of the specimen.
•
Focus Exposure:
The exposure time during focusing, it can have three states:
o
Short
o
Normal
o
Long
Sample detection
The Sample detection panel offers options for setting threshold and the selection of scannable
areas.
•
Source
o
Brightfield preview
–
a normal type of illumination in the microscope for scanning
the slide. This option is recommended when scanning in Brightfield mode.
o
Pre-scan
–
creates a fast FL preview image that is displayed in the Profile Wizard
window. Scanning process takes a couple of minutes only, and it cannot be
interrupted.
o
Pre-scan high-resolution
–
creates a high-resolution fast FL preview image of
every FOV within the selected area (in single layer DAPI, with an illumination time
of 20ms) that is displayed in the Profile Wizard window. Scanning process takes a
couple of minutes only and cannot be interrupted.