Pannoramic MIDI II 2.0.5 User’s Guide
5.1.1 Profile Settings Panel
Multilayer mode
•
Select
Single layer
option if you want to scan a single layer of the slide.
•
By choosing the
Extended focus
option, the software selects the sharpest image from each
focus level for each image field, and combines them into one single image. Therefore those
parts that were blurry in auto/manual focus will also be sharp. This method guarantees the
maximum depth of sharpness, however, it reduces digitization speed.
Figure 23 – Focus level and step size
•
Z-stack:
Both for
Extended focus
and
Z-Stack
scanning methods, you can determine the
desired number of focus levels with the
Levels
selection box. The current focus value is the
starting point. If you set
5
for example, one current, two focus levels above and below FOVs
are captured. Therefore, it is advised to choose an odd number for the focus levels. Separate
layers are created and stored this way, and can be opened with Pannoramic Viewer. The Z-
stack slide contains the extended focus layer.
Step size
determines the spacing between focus levels. One step is around 0.2 µm.
Default settings are:
Levels
: 7;
Step size
: 5.
December 7, 2018 – Rev. 1
3DHISTECH Ltd.
49(144)