Waters 1500-Series, Руководство оператора

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4-16 Preparing for Operation
If you did not yet create a chromatographic system with the 1500-series
pump, do so now.
•
Ensure the pump is properly configured in the Empower Node
Properties, which allows you to scan for available instruments
(including the pump), and set the pump-head size in the
Instruments tab (for 1525µ and 1525EF only).
• Bring the system online.
For detailed instructions, refer to the Empower 2 online Help.
4. In the Navigation pane, click Run Samples.
5. Select the system and click OK.
6. In the Run Samples window, click the Start Flow icon , and enter
appropriate values for the pump’s flow rate and gradient settings.
Tip:
To quickly prime and purge just the pump,
• for the 1515 pump, set the rate to 2.0 mL/min.
• for the 1525 pump, set the rate to 4.0 mL/min and gradient to 50%
A, 50% B.
• for the 1525µ pump
,
set the rate to 2.0 mL/min and gradient to 50%
A, 50% B.
• for the 1525EF pump, set the rate to 6.0 mL/min and gradient to
50% A, 50% B.
7. Select Immediate Flow Change and click OK.
8. Allow the pump to run for approximately one minute, and then verify
that the eluent flow exiting to waste is normal and consistent for the
specified flow rate.
9. Click the Stop Flow icon in the Run Samples window to stop the
flow.
Purging the flow path via Empower 2 control
Purging ensures that all eluent in the flow path is replaced with new eluent
before you equilibrate the system and run samples (see page 4-2 ).