Waters 1500-Series, Руководство оператора

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4-18 Preparing for Operation
Equilibrating the system via Empower 2 control
Equilibrate the system to achieve a final state of system readiness for running
samples (see page 4-2 ).
Before equilibrating
• Make sure the eluent is filtered and degassed.
• Allow the eluent and the system to reach proper temperatures before
pump operation.
• Prime the pump, as described in the section above.
• Ensure the pump’s reference valve is turned clockwise to the closed
position, directing the eluent flow to the system (for the 1525µ pump,
position the vent valve knob to the system).
To equilibrate the system:
1. Determine flow rate and gradient settings appropriate for your column
and application, and then follow step 1 through step 7 on page 4-15 .
2. Allow the system to equilibrate for several minutes.
Tip:
The amount of running time required for equilibration depends on
total system volume, eluent miscibility, flow rate, and so on.
3. Monitor the system pressure to verify it is normal and stable. Inspect
the eluent flow exiting the system to verify it is normal and consistent
for the specified flow rate.
Tip:
To monitor the detector baseline during system equilibration, select
an instrument method for the pump and click Monitor. For information
about creating an instrument method used for equilibration, refer to the
Empower 2 online Help.
4. Click the Stop Flow icon in the Run Samples window to stop the
flow.