Waters 1500-Series, Руководство оператора

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Startup and initial preparation 4-9
The seal wash solvent lubricates the plunger. It also flushes away solvent or
precipitated salts forced past the plunger seal from the high-pressure side of
the solvent piston chamber.
Tips:
• Once you install the seal wash option, use it at all times during
instrument operation to protect plungers and seals.
• For reversed-phase HPLC applications, use an aqueous plunger seal
wash solution with enough organic content to inhibit bacterial growth.
For example, use a 4:1 water methanol or water acetonitrile solution,
depending on your application.
• For all GPC separations, use a 1:1 water methanol seal wash solution. If
required, use an intermediate solution to prevent immiscibility or
precipitation problems when you switch between GPC and
reversed-phase analytical solvents.
Priming the plunger seal wash pump
You must prime the plunger seal wash pump for first-time use or when the
entire line is dry.
Ensure the plunger seal wash supply line (labeled “Seal Wash In”) is in the
plunger seal wash solvent bottle and that the plunger seal wash waste line
(clear) is in an appropriate waste container.
To prime the seal wash pump:
1. Fill the plunger seal wash solvent bottle with an appropriate solvent.
2. Place the plunger seal wash supply line in the plunger seal wash solvent
bottle.
3. Connect the tubing adapter to the syringe provided with the kit.
4. Disconnect the seal wash outlet tube from the waste tubing.
5. Attach the syringe to the seal wash outlet tube, and pull out the syringe
plunger to create vacuum in the system.
6. Power-on the pump.
Caution:
To avoid contamination issues, never recycle seal wash.