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6-10
Troubleshooting
Reduced retention
times
Incorrect flow rate
Change flow rate.
Incorrect eluent
composition
Change composition.
High column temperature Reduce column
temperature.
Incorrect eluent
Use correct eluent.
Column contaminated
Clean or replace column.
Incorrect column
Use correct column.
Eluent not properly
degassed
Degas eluent.
Reproducibility
errors
Eluent not properly
degassed
Degas eluent.
Incorrect
chemistry/integration
Verify
chemistry/integration.
Baseline drift,
rapid
Column not equilibrated
Equilibrate column.
Eluent contaminated
Use fresh eluent.
Eluent not properly
degassed (rapid or slow
drift)
Degas eluent.
Flow fluctuations (rapid or
slow drift)
Fix pump problems,
replace pump seals and
check valves.
Baseline drift, slow Eluent contaminated
Use fresh eluent.
Ambient temperature
fluctuations
Stabilize operating
environment temperature
enough to allow full
equilibration.
Correcting chromatographic problems (Continued)
Symptom
Possible cause
Corrective action
Содержание 1500-Series
Страница 14: ...xiv Table of Contents ...
Страница 32: ...1 18 Introduction ...
Страница 50: ...2 18 Installing the HPLC Pump ...
Страница 84: ...3 34 Installing Options and Accessories ...
Страница 108: ...4 24 Preparing for Operation ...
Страница 160: ...A 14 Safety Advisories ...
Страница 184: ...C 16 Solvent Considerations ...