Waters 1500-Series, Руководство оператора

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Wavelength selection C-13
Mixed mobile phases
The following table provides approximate wavelength cutoffs for some other
solvents, buffers, detergents, and mobile phases. The solvent concentrations
represented are those most commonly used. If you want to use a different
concentration, you can determine approximate absorbance using Beer’s Law,
since absorbance is proportional to concentration.
Cyclohexane 200 n -Pentane 190
Cyclopentane 200 n -Propanol 210
Diethyl amine 275 n -Propyl chloride 225
Dioxane 215 Nitromethane 380
Ethanol 210 Petroleum ether 210
Ethyl acetate 256 Pyridine 330
Ethyl ether 220 Tetrahydrofuran 230
Ethyl sulfide 290 Toluene 285
Ethylene dichloride 230 Xylene 290
Wavelength cutoffs for different mobile phases
Mobile phase
UV
cutoff
(nm)
Mobile phase
UV
cutoff
(nm)
Acetic acid, 1% 230 Sodium chloride, 1 M 207
Ammonium acetate,
10 mM
205 Sodium citrate, 10 mM 225
Ammonium bicarbonate,
10 mM
190 Sodium dodecyl sulfate 190
BRIJ 35, 0.1% 190 Sodium formate, 10 mM 200
CHAPS, 0.1% 215 Triethyl amine, 1% 235
Diammonium phosphate,
50 mM
205 Trifluoracetic acid, 0.1% 190
UV cutoff wavelengths for common chromatographic solvents (Continued)
Solvent
UV cutoff
(nm)
Solvent
UV cutoff
(nm)