5. Mount the combination carefully in the injection block (
Figure 16–17: Mounting of the fused
silica connector in the VT-03 micro flow cell (Page 173)
6. Let the fused silica protrude slightly (< 0.5 mm) through the injection hole.
7. Clean the factory-supplied glass mounting plate from particles.
8. Carefully push the block on the glass plate until the silica capillary is flush with the surface.
9. Fix the fused silica capillary firmly with the finger-tight while keeping a slight pressure on
the block against the glass plate.
10. Mount the two flow cell blocks by crosswise tightening the bolts (max. 13 Ncm).
Figure 16–18: Different column connectors with micro flow cell
Column type A has no ferrule but ends with fused silica (1); column type B has a ferrule
connector (1).
On the left (A), the standard installation with capillary connector (2) is shown. This is
suitable for fused silica columns. On the right (B), installation with a ferrule and sleeve
connected to the column is shown.
11. Connect the other end of the capillary connector as shown in "Different column connectors
with micro flow cell" figure. Use a ferrule with sleeve, or couple to the capillary column
using the connector sleeve. If leakage occurs, a low-dead coupler can be used with finger-
tights and sleeve.
12. Continue installation as described at point 3 in
1 (skip point 3) in
(salt bridge REF), depending on the type of reference
electrode used.
Note:
To speed up the filling of the micro flow cell, the cell can be connected directly to the
HPLC, thus bypassing the column.
Figure 16–19: Filling a VT-03 micro flow cell at 200–400 µL/min using a low dead-
volume connector (1); pressure must not rise > 200 bar
December 16, 2021, 715007395 Ver. 00
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