Warning:
Applying low working volume settings should be done with great care
because it may cause excessive pressure buildup over the flow cell, excessive
baseline noise, and damage to the cell. Do not operate the cell at position 0.
15.3.3 Installation in LC system
Prior to installation of the SenCell, ensure that the following precautions are followed.
1. For optimal performance, all metal parts in your HPLC system should be passivated with
30% Phosphoric Acid.
2. Before connecting a new column, read the manufacturer’s instructions. Our experience is
that thorough preconditioning of a column is always required. Only a preconditioned
column is electrochemically clean. Otherwise, the background current may be
unacceptably high and substantial fouling of the working electrode may occur. For reverse-
phase columns, flushing with 50% methanol in water for three days at a low flow rate is
highly recommended.
3. Before connecting the flow cell, ensure that the LC system with column is well equilibrated
with mobile phase prepared using high-purity chemicals.
Notice:
If an ISAAC reference electrode is used, ensure that the mobile phase
contains at least 2 mmol/L chloride (KCl or NaCl) ions.
4. Passage of air bubbles through the flow cell will lead to unacceptable noise levels and
spikes. The use of an in-line degasser is therefore strongly recommended. A one-time
degassing step for the HPLC buffer is almost never sufficient.
15.3.4 Installing the SenCell
Follow this procedure to install the SenCell.
To install the SenCell:
1. If applicable, install the SenCell clamp (700013160) from the startup kit in the center
position of the 3465 Detector with a Phillips screwdriver.
2. Connect the column to the flow cell inlet with 1/16-inch OD small-bore PEEK tubing (0.3-
mm ID or smaller depending on the column bore size) using the PCTFE 10-32 finger-tight
fitting (700013151). Use only factory-supplied finger-tights in the flow cell; others may
cause serious damage.
Let the tubing protrude about 1.5 cm from the finger-tight fitting and tighten it such that the
tubing is not or only slightly indented by the fitting. Do not over-tighten the finger-tight
fitting. Over-tightening affects the flow pattern through the tubing and may strongly
decrease flow cell performance.
December 16, 2021, 715007395 Ver. 00
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