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Instructions for Use

12 Glossary of Terms and Abbreviations

ANSI

American National Standards Institute

Deepwell Plate

Plate with an SBS footprint featuring 48, 96 or 384

wells with a larger volume than microplates

DWP

Deepwell plate

EMC

Electro-Magnetic Compatibility

Incubate

Keeping an organism, cell or cell culture at the

optimum temperature for growth and development

Microtiter Plate

Plate with an SBS footprint featuring 24, 48, 96 or

384 wells

Mixing Load

All samples to be mixed located in their respective

tubes or plates

MTP

Microtiter plate

PCR

Polymerase Chain Reaction

Pellet

A small densely packed mass. Created, for

example, via the centrifugation of a suspension

PPE

Personal Protective Equipment

Re-suspending

Dissolving a pellet by Vortexing in a liquid with the

material being redistributed in the liquid

RPM

Revolutions Per Minute

SBS

Society for Bio molecular Screening

Semi-skirted PCR
Plate

PCR plate with an outer surrounding half edge

Skirted PCR Plate

PCR plate with an outer surrounding edge

Un-skirted PCR Plate

PCR plate without an outer surrounding edge

Vortexing

Strong whirling or spinning flow of liquid by manually

pressing a tube onto the Vortexing pad

Well

A single cavity in a Microtiter plate, PCR plate or

Deepwell plate

Содержание Co-mix

Страница 1: ...Instructions for Use...

Страница 2: ...nge without notice ITL assumes no responsibility for any errors that may appear in this or related documentation Document V501001 Revision 3 1412 Vitl is the brand name for Integrated Technologies own...

Страница 3: ...result in severe injury or death WARNING Indicates a hazardous situation which could if not avoided result in severe injury or death or severely damage the unit CAUTION Indicates a hazardous situation...

Страница 4: ...empting to operate the unit Improper use of this unit or its accessories may impair their functionality and invalidate the manufacturer s warranty Unit Handling Precautions CAUTION Care should be take...

Страница 5: ...y earthed mains supply with appropriate earth leakage and over current protection WARNING Always ensure that the mains power connector is securely inserted into the rear of the unit and any excess pow...

Страница 6: ...when mixing and vortexing WARNING Injury can occur from flying plates and tubes if they are not correctly inserted or if the maximum recommended mixing speed or total load weight is exceeded Always e...

Страница 7: ...injury or death DANGER The externally accessible unit fuse will only blow under an extreme internal fault condition This fuse should only be changed after the unit has been thoroughly inspected by a...

Страница 8: ...10 1 2010 EN 61010 2 051 2003 UL 61010 1 2001 2nd Edition CAN C22 2 CSA 61010 1 EMC EN 61326 2006 Class B FCC CFR 47 Parts 15 107 and 15 109 Class B RoHS and WEEE Directive Compliance This product is...

Страница 9: ...terface 6 1 Plate Mixer Plate Holder 6 3 Plate Clamping Lever 6 3 2 Tube Vortexer 6 4 Unit Label and Serial Number 4 Mains Power Switch 5 Mains Power Inlet 5 External Fuse Access Cover 8 1 Mains Power...

Страница 10: ...lowing accessories Table 3 Unit Accessories Mains power cord varies according to country of use 5 11 96 well PCR plate and 0 2ml tube adaptor block 6 3 6 3 3 24 well 0 5ml tube adaptor block 6 3 6 3 4...

Страница 11: ...ions listed in section 2 Install and test the Co mix unit using the following procedure 1 Place the Co mix unit on the suitably selected working surface as specified above ensuring that the ventilatio...

Страница 12: ...LCD touchscreen with page selection tabs down the left hand side of the display and function buttons along the bottom Page Selection Tabs Control and Status Function and Option buttons The Co mix is...

Страница 13: ...he program s ID and the assigned speed and time parameters Program ID Favourite Pre defined Speed Time To load a program use the Home button to jump to the top of the program grid list or the Next and...

Страница 14: ...us menu Add remove Favourite program 7 1 1 New Edit or Delete program 7 1 Change program ID or Info 7 1 3 Change user program Adaptor type 7 1 3 Unlock program speed and time 7 1 3 Increase or Decreas...

Страница 15: ...matic Tube Vortexing 6 4 1 Manual Tube Vortexing 6 4 1 Batch Tube Vortexing 6 4 2 Add favourite program 7 1 1 Create new program 7 1 2 Edit user defined program 7 1 3 Change program speed and time 7 1...

Страница 16: ...nform to the ANSI SBS Standard for Microplates to ensure secure positioning in the plate holder The maximum permissible mixing speed for DWPs and Adaptor Blocks or other plates over 80g is 2000 RPM Th...

Страница 17: ...immediate needs The mixer speed can be set between 200 RPM and 3000 RPM and the time between 5 seconds and 28 days Home Previous Next Tools Accept To load the MIX program select the Mixer page tab the...

Страница 18: ...MIX program the Increase and Decrease buttons are always enabled to allow the speed and time parameters to be freely changed even when the mixer is running Place the plate or adaptor block vertically...

Страница 19: ...ll automatically close to firmly grip the plate or adaptor block and the specified mixing program will be run The mixing timer will now count down each second until it reaches zero or the Stop button...

Страница 20: ...orarily disabled by pressing the Mute button The display will return to the previous screen ready to accept the next plate to be mixed Pressing the Programs button will close the plate clamping mechan...

Страница 21: ...0 30 5 to 50 x PCR 96 96 well PCR plate 0 2ml tubes or strips 1650 30 5 to 50 1 MTP 384 384 well MTP 2000 15 10 to 60 x DWP 384 384 well DWP 2000 15 10 to 60 x PCR 384 384 well PCR plate 2600 15 10 to...

Страница 22: ...ined variants of the pre defined programs Place the plate or adaptor block into the plate holder making sure it sitting flat on the base of the holder and then press the Start button to close the clam...

Страница 23: ...suring that they are securely and fully pushed down in the wells 3 Insert the adaptor block into the plate holder on the unit 4 After mixing remove the adaptor block from the unit and then remove the...

Страница 24: ...m improper vortexing of tubes Never vortex open topped tubes Always use sealed tubes Never vortex tubes made of glass or other fragile materials Not suitable for flasks or other flat bottomed vessels...

Страница 25: ...Mute Start To vortex a tube simply press the tube down onto the centre of the vortexer pad and vortexing will automatically start at the programmed speed The timer will count down each second and vort...

Страница 26: ...when next required See sections 6 4 3 and 7 3 for details The unit clicks once to acknowledge when the tube has been pressed onto the vortexer pad and beeps once when the programmed time has elapsed T...

Страница 27: ...pressure so that there is still sufficient contact with the pad to maintain the vortexing action but without being unduly strenuous on the user If desired this mode can be permanently enabled for all...

Страница 28: ...ms the user can create variants of the existing ones This allows new programs to be developed and optimised for the specific plate type sample assay and fill volume being used Home Previous Next Tools...

Страница 29: ...se will be regularly used Thus to help organise the full list preferred programs can be added to a favourites list which always appears at the top of the program selection list To add a program to the...

Страница 30: ...available when Protected mode is enabled See selection 7 4 4 for details This will create a new copy of the selected program and pop up the program ID editing keyboard Shift Delete Cancel Left Right A...

Страница 31: ...unctions Exit ID Info Adaptor Unlock These editing functions allow the program ID Info text and associated Adaptor block type to be changed as described in Table 11 Table 11 Program Editing Functions...

Страница 32: ...eing used Any changes to these parameters are automatically saved to memory for next time Pressing the Profile button brings up the advanced program profiling menu which allows a sequence of up to 20...

Страница 33: ...Profile Step Parameters Parameter Purpose Range RPM Mixing speed Automatically limited to 2000 RPM when the program specifies the use of an adopter block 200 to 3000 RPM TIME Mixing step duration spe...

Страница 34: ...sted using the Increase and Decrease buttons These parameters can only be altered via the Profile button To create a new profiled program first create a new program as described in section 7 1 2 above...

Страница 35: ...rsor Up one line Move profile step cursor Down one line Modify profile step parameters at cursor position Switch to menu functions Set 2 Set 2 Switch back to menu functions Set 1 Cut and remove profil...

Страница 36: ...ept 4 Enter the new SECS value followed by Accept 5 Press Accept again skips RAMP When all six values had been accepted the new settings are saved and the display returns to the previous profile editi...

Страница 37: ...ste Modify 7 3 Vortexer Program Tools The Co mix can store up to 99 user defined vortexing programs allowing specific programs to be developed and optimised for different tube types sample solutions a...

Страница 38: ...in the more detail in the following sub sections Table 15 User Preferences and Options Beeper volume Muted Low Medium High 7 4 1 Touchscreen button click volume Disabled Quiet Loud 7 4 1 LCD backligh...

Страница 39: ...ditions 7 4 3 Cooling Fan Operating Mode The unit s internal cooling fan draws air in from around the plate holder and gently blows warm air out of the ventilation slots in the base of the unit It is...

Страница 40: ...ation If processing large batches of tubes it may be desirable for the vortexer to run on after the tube has been removed This auto Restart mode can be permanently enabled for all vortexer programs by...

Страница 41: ...nly be replaced by a suitably qualified engineer DANGER The unit fuse will only blow as a result of an internal unit fault This fuse should only be changed after the unit has been thoroughly inspected...

Страница 42: ...nism 6 Clean around the gap between the plate holder and casework to remove any build up of debris avoiding pushing it into the unit 7 Clean along the spill gutter strip at the rear of the unit 8 Clea...

Страница 43: ...oroughly clean all outside surfaces of the product including any accessories power cords manuals packaging etc with a damp cloth soaked with suitable disinfectant solution such as Virkon Allow to dry...

Страница 44: ...e completely dry and free of any condensation before being packed 8 5 Product Disposal At end of life this product must be disposed of in accordance with your local authority regulations for the dispo...

Страница 45: ...ed for optimum performance 6 3 1 Mixing time too short Increase mixing time to ensure adequate mixing 6 3 1 Lid wetting or sample leakage Reduce mixing speed or fill volume 6 3 Vortexer Does Not Opera...

Страница 46: ...assistance 8 E23 E24 Mixer overloaded or jammed Switch off unit and inspect plate holder for obstructions 8 2 E25 Mixing load unbalanced Ensure unit correctly installed on solid working surface 5 Ensu...

Страница 47: ...r or peripheral not supplied specified or sanctioned by ITL will invalidate this warranty This warranty is provided to the original purchaser of the product for one year from the date of purchase Unde...

Страница 48: ...0V 20x5mm Operating Environment Temperature Range Relative Humidity Range Maximum Operating Altitude 10 to 38 C 20 to 85 non condensing 2000 m above sea level Storage and Transportation Temperature Ra...

Страница 49: ...es MTP Microtiter plate PCR Polymerase Chain Reaction Pellet A small densely packed mass Created for example via the centrifugation of a suspension PPE Personal Protective Equipment Re suspending Diss...

Страница 50: ...ing Programs 27 7 Advanced Set up Features 28 7 1 Mixer Program Tools 28 7 1 1 Favourite Programs List 29 7 1 2 Creating a New User defined Program 30 7 1 3 Changing the Program ID Info Adaptor Type a...

Страница 51: ...Menu Structure and Button Presses 15 Table 7 Supported Plate and Tube Types 16 Table 8 Pre defined Mixing Programs 21 Table 9 Supported Vortexer Tube Types 24 Table 10 Program Tools Options 29 Table...

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