ThermoFisher Scientific Vanquish VH-D10, Руководство по эксплуатации

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5 • Installation
Vanquish Diode Array Detectors (VH-D10) Operating Manual Page 73
5.7 Determining the Backpressure in the Flow Cell
All modules, capillaries and waste lines downstream of the flow cell
contribute to the backpressure on the flow cell. If you attach other
modules (such as detectors or a mass spectrometer) in the flow path
after the flow cell, make sure that the pressure within the flow cell does
not exceed 6 MPa.
NOTICE—Sensitive LightPipe Flow Cells
LightPipe flow cells are highly sensitive. Improper use or handling can
lead to increased noise, increased drift, increased refractive index
sensitivity, clogging, leaks on the flow cell, or even destruction of the
flow cell. Handle LightPipe flow cells always with care and use them only
and strictly within their specifications of up to 6 MPa and 50 °C.
When
•
If you install additional devices such as detectors, a mass
spectrometer and capillaries in the flow path after the flow cell:
♦
Before installing the mentioned additional devices
♦
After having installed the mentioned additional devices
•
Before you use a new method
•
After you have replaced capillaries in the flow path after the flow
cell
•
Regularly, because of the aging process of the capillaries even if
nothing of the above has occurred.
Parts required
• Viper union, for example, from the system ship kit
• Capillaries and waste line from the detector ship kit
• One of the following solvent mixtures of high viscosity:
♦ If using gradients :
♦ Solvent to be used in your application.
♦
Use the mixing ratio with the highest viscosity.
♦ If not using gradients
:
♦
60% water and 40% methanol
–or–
♦
70% water and 30% acetonitrile