ThermoFisher Scientific Vanquish VH-D10, Руководство по эксплуатации

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7 • Maintenance and Service
Page 134 Vanquish Diode Array Detectors (VH-D10) Operating Manual
7.8 Back-Flushing the Flow Cell
If a LightPipe flow cell shows increased backpressure or even blockage,
back-flushing the flow cell at a high flow rate can help to regain the
original performance of the flow cell. This can also be performed as a
remedy if the light transmittance of the flow cell is reduced due to
contamination of the light pipe, causing increased noise and instable
baseline.
To prevent damage to the flow cell, perform the back-flushing
procedure only with the back-flush kit.
With the back-flush capillary, the pressure at the flow cell can be limited
securely to the specified pressure range, even with blockages in the flow
cell. The pressure that is applied to the flow cell is limited by a bypass
flow through an overpressure line during the back-flush procedure. An
in-line filter on the back-flush capillary prevents contamination of the
flow cell or the overpressure line.
To remove the blockage (all particles and contamination) from the flow
cell, it may be required to repeatedly flush the flow cell in alternating
directions (backward and forward).
When
• Increased backpressure in the flow cell
• Blockage occurred in the flow cell
• Decreased optical transmission of the flow cell
Parts required
•
Back-flush kit for LightPipe flow cells
Figure 31: Back-flush kit