126
iSort
™
Automated Cell Sorter User Guide
Appendix B: Technical specifications
Note:
Technical specifications of the iSort
™
Automated Cell Sorter are subject to change without notice. For
the latest product information, see the product page at
Physical
characteristics
Dimensions (H × D × W):
49 × 41 × 67 cm (19 × 16 × 26.5 inches)
Weight:
41 kg (89 lb)
Footprint:
Approximately 92 cm × 92 cm (36 in × 36 in). With the fluidics
compartment door completely open, the entire system requires a total bench
width of 135 cm (53.2 in).
Operating temperature range:
18
−
25°C (64
−
77°F)
Storage temperature range:
5
−
35°C (41
−
95°F)
Operating humidity:
20–70% non-condensing
Operating power:
100–240 VAC, 4.0 A
Frequency:
50–60 Hz
Optics
Laser:
488-nm (blue), 165 mW
Optical detection:
SSC (488/10) and GFP (525/50BP), Photomultipliers (PMT)
Fluidics
Nozzle:
Ceramic tip, 85-µm orifice
Sample sort rate:
Up to 12,000 events/second
Sample flow rate:
23 µL/minute (fixed)
Maximum run time:
~4 hours (limited by sheath volume)
Hardware
Biohazard containment:
Fits in any standard >4-foot biosafety cabinet. Additional
modifications/filters may be required to meet individual institutional safety
requirements.
Output ports:
3 ports, USB 2.0
Networking capability:
Connection through Windows/SMB network via an
Ethernet cable connection.
Power supply:
AC adapter with country
‐
specific power cords.
Sample
Cell type:
Mammalian
Sample sort direction:
One- or Two-way
Sample input vessel:
12 × 75-mm tube (5 mL)
Sort collection vessel:
1.5 mL, 5 mL, 50-mL tubes
Minimum sample volume
: 200 µL
Separation limit
: ~2–20 µM diameter using a 85 µM nozzle
Содержание invitrogen iSort Series
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